ArticleRNA (New York, N.Y.)2007
Depletion of newly synthesized Argonaute1 impairs the RNAi response in Trypanosoma brucei.
Article in RNA (New York, N.Y.), 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 18 citations in OpenAlex.
- Article
- Comparative transcriptome analysis of small noncoding RNAs in different stages of Trypanosoma brucei.RNA (New York, N.Y.) · 2013Article
- Pseudogene-derived small interference RNAs regulate gene expression in African Trypanosoma brucei.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Small nucleolar RNA interference in Trypanosoma brucei: mechanism and utilization for elucidating the function of snoRNAs.Nucleic acids research · 2010Article
- RNA interference in Trypanosoma brucei: role of the n-terminal RGG domain and the polyribosome association of argonaute.The Journal of biological chemistry · 2009Article
- Distinct and overlapping roles for two Dicer-like proteins in the RNA interference pathways of the ancient eukaryote Trypanosoma brucei.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Using morpholinos for gene knockdown in Giardia intestinalis.Eukaryotic cell · 2009Article
- RNA interference in infectious tropical diseases.The Korean journal of parasitology · 2008Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Trypanosoma brucei, Argonaute1 (TbAGO1) is an essential component of the RNA interference (RNAi) pathway. While characterizing a TbAGO1 conditional knockout cell line, we discovered that, upon blockage of TbAGO1 transcription, the RNAi response to transfected double-stranded RNA was severely inhibited, although there was no change in the TbAGO1 protein level. This observation suggested that steady-state TbAGO1 was not sufficient to fully support the RNAi response to transfected dsRNA and implicated newly synthesized Argonaute in this phenomenon. Indeed, a translational blockade of TbAGO1 mRNA with an antisense morpholino oligonucleotide resulted in inhibition of the RNAi response, even though the steady-state level of TbAGO1 remained unchanged during the time of the assay. Thus, we concluded that in T. brucei, newly synthesized TbAGO1 is required to support an efficient RNAi response. We speculate that newly processed siRNAs may be preferentially loaded onto newly synthesized TbAGO1, and this mechanism may contribute to the homeostasis of the RNAi pathway.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.