ArticleBiophysical journal2007
Establishing a definitive stoichiometry for the Na+/monocarboxylate cotransporter SMCT1.
Article in Biophysical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed.
- MicroRNAs and Their Inhibition in ModulatingInternational journal of molecular sciences · 2025Article
- Human Glucose Transporters in Health and Selected Neurodegenerative Diseases.International journal of molecular sciences · 2025Review
- Time-restricted feeding mitigates Alzheimer's disease-associated cognitive impairments via aiMeta · 2025Article
- Genome-Wide Analysis Reveals Expansion and Positive Selection of Monocarboxylate Transporter Genes Linked to Enhanced Salinity and Ammonia Tolerance inAnimals : an open access journal from MDPI · 2025Article
- Bidirectional Regulation of Sodium Acetate on Macrophage Activity and Its Role in Lipid Metabolism of Hepatocytes.International journal of molecular sciences · 2023Article
- Microbiota-microglia connections in age-related cognition decline.Aging cell · 2022Review
- Human Glucose Transporters in Renal Glucose Homeostasis.International journal of molecular sciences · 2021Review
- Sodium Acetate Inhibit TGF-β1-Induced Activation of Hepatic Stellate Cells by Restoring AMPK or c-Jun Signaling.Frontiers in nutrition · 2021Article
- EmrE reminds us to expect the unexpected in membrane transport.The Journal of general physiology · 2020Article
- Involvement of butyrate in electrogenic KPflugers Archiv : European journal of physiology · 2019Article
- Distribution of glucose transporters in renal diseases.Journal of biomedical science · 2017Review
- Evidence for a Revised Ion/Substrate Coupling Stoichiometry of GABA Transporters.The Journal of membrane biology · 2015Article
- The transport mechanism of the human sodium/myo-inositol transporter 2 (SMIT2/SGLT6), a member of the LeuT structural family.American journal of physiology. Cell physiology · 2014Article
- Nutrient transport in the mammary gland: calcium, trace minerals and water soluble vitamins.Journal of mammary gland biology and neoplasia · 2014Review
- A novel delta current method for transport stoichiometry estimation.BMC biophysics · 2014Article
- The Concise Guide to PHARMACOLOGY 2013/14: transporters.British journal of pharmacology · 2013Article
- Intracellular Ca2+ modulation during short exposure to ischemia-mimetic factors in isolated rat ventricular myocytes.Collegium antropologicum · 2009Article
- The ABCs of membrane transporters in health and disease (SLC series): introduction.Molecular aspects of medicineReview
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5 authors.
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Abstract
Several different stoichiometries have been proposed for the Na(+)/monocarboxylate cotransporter SMCT1, including variable Na(+)/substrate stoichiometry. In this work, we have definitively established an invariant 2:1 cotransport stoichiometry for SMCT1. By using two independent means of assay, we first showed that SMCT1 exhibits a 2:1 stoichiometry for Na(+)/lactate cotransport. Radiolabel uptake experiments proved that, unlike lactate, propionic acid diffuses passively through oocyte membranes and, consequently, propionate is a poor candidate for stoichiometric determination by these methods. Although we previously determined SMCT1 stoichiometry by measuring reversal potentials, this technique produced erroneous values, because SMCT1 simultaneously mediates both an inwardly rectifying cotransport current and an outwardly rectifying anionic leak current; the leak current predominates in the range where reversal potentials are observed. We therefore employed a method that compared the effect of halving the external Na(+) concentration to the effect of halving the external substrate concentration on zero-current potentials. Both lactate and propionate were cotransported through SMCT1 using 2:1 stoichiometries. The leak current passing through the protein has a 1 osmolyte/charge stoichiometry. Identification of cotransporter stoichiometry is not always a trivial task and it can lead to a much better understanding of the transport activity mediated by the protein in question.
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