ArticleGene therapy2007
Transient activation of transgene expression by hydrodynamics-based injection may cause rapid decrease in plasmid DNA expression.
Article in Gene therapy, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 41 citations in OpenAlex.
- Epigenetic regulation of transgenes.Journal of biotechnology · 2026Review
- HDI-STARR-seq: Condition-specific enhancer discovery in mouse liver in vivo.BMC genomics · 2024Article
- Article
- Construction of pVAX-1-based linear covalently closed vector with improved transgene expression.Molecular biology reports · 2024Article
- Mechanistic Insights in NeuroD Potentiation of Mineralocorticoid Receptor Signaling.International journal of molecular sciences · 2019Article
- Contribution of Epigenetic Modifications to the Decline in Transgene Expression from Plasmid DNA in Mouse Liver.Pharmaceutics · 2015Article
- A designed curved DNA sequence remarkably enhances transgene expression from plasmid DNA in mouse liver.Gene therapy · 2012Article
- Efficacy and safety of Sleeping Beauty transposon-mediated gene transfer in preclinical animal studies.Current gene therapy · 2011Review
- Inhibition of nuclear delivery of plasmid DNA and transcription by interferon γ: hurdles to be overcome for sustained gene therapy.Gene therapy · 2011Article
- Positive correlation between the generation of reactive oxygen species and activation/reactivation of transgene expression after hydrodynamic injections into mice.Pharmaceutical research · 2011Article
- Probing the in vitro mechanism of action of cationic lipid/DNA lipoplexes at a nanometric scale.Nucleic acids research · 2011Article
- Duration of expression and activity of Sleeping Beauty transposase in mouse liver following hydrodynamic DNA delivery.Molecular therapy : the journal of the American Society of Gene Therapy · 2010Article
- Quantification of plasmid DNA copies in the nucleus after lipoplex and polyplex transfection.Journal of controlled release : official journal of the Controlled Release Society · 2009Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The intranuclear disposition of exogenous DNA is quite important for the therapeutic effects of the administered DNA. The expression efficiency from one copy of exogenous DNA delivered by hydrodynamics-based injection dramatically decreases over time, and this 'silencing' occurs without CpG methylation. In this study, naked luciferase-plasmid DNA was delivered into mouse liver by hydrodynamics-based injection, and modifications of the histones bound to the plasmid DNA were analyzed by a chromatin immunoprecipitation (ChIP) analysis. In addition, the effects of a second hydrodynamics-based injection on the expression from the plasmid DNA were examined. The ChIP analysis revealed that the modification status of histone H3 remained constant from 4 h to 4 weeks. Surprisingly, the injection of saline without DNA enhanced the luciferase expression from the preexisting DNA administered 4 and 14 days previously. Our results suggest that histone modification plays no role in the silencing. Instead, our data suggest that the transgene expression is activated by the hydrodynamics-based injection manipulation, and that the return from the activated status causes the silencing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.