ArticleBMC genomics2007
Diverse histone modifications on histone 3 lysine 9 and their relation to DNA methylation in specifying gene silencing.
Article in BMC genomics, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 44 citations in OpenAlex.
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- Single-cell multimodal analysis in a case with reduced penetrance of Progranulin-Frontotemporal Dementia.Acta neuropathologica communications · 2021Article
- Epigenome signatures landscaped by histone H3K9me3 are associated with the synaptic dysfunction in Alzheimer's disease.Aging cell · 2020Article
- Environmental pollution and DNA methylation: carcinogenesis, clinical significance, and practical applications.Frontiers of medicine · 2015Review
- Effects of trichostatin A on In vitro development and DNA methylation level of the satellite I region of swamp buffalo (Bubalus bubalis) cloned embryos.The Journal of reproduction and development · 2014Article
- Characterization of DNA methylation and its association with other biological systems in lymphoblastoid cell lines.Genomics · 2012Article
- Valproic acid improves the in vitro development competence of bovine somatic cell nuclear transfer embryos.Cellular reprogramming · 2012Article
- Concomitant heterochromatinisation and down-regulation of gene expression unveils epigenetic silencing of RELB in an aggressive subset of chronic lymphocytic leukemia in males.BMC medical genomics · 2010Article
- Epigenetic regulation of hepatic endoplasmic reticulum stress pathways in the ethanol-fed cystathionine beta synthase-deficient mouse.Hepatology (Baltimore, Md.) · 2010Article
- Integrating the multiple dimensions of genomic and epigenomic landscapes of cancer.Cancer metastasis reviews · 2010Review
- Genome-wide analysis of histone H3 lysine 27 trimethylation by ChIP-chip in gastric cancer patients.Journal of gastroenterology · 2009Article
- Article
- An integrative genomic and epigenomic approach for the study of transcriptional regulation.PloS one · 2008Article
- Mammalian DNA methyltransferases: a structural perspective.Structure (London, England : 1993) · 2008Review
- Intersection of nuclear receptors and the proteasome on the epigenetic landscape.Environmental and molecular mutagenesis · 2008Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundPrevious studies of individual genes have shown that in a self-enforcing way, dimethylation at histone 3 lysine 9 (dimethyl-H3K9) and DNA methylation cooperate to maintain a repressive mode of inactive genes. Less clear is whether this cooperation is generalized in mammalian genomes, such as mouse genome. Here we use epigenomic tools to simultaneously interrogate chromatin modifications and DNA methylation in a mouse leukemia cell line, L1210.
resultsHistone modifications on H3K9 and DNA methylation in L1210 were profiled by both global CpG island array and custom mouse promoter array analysis. We used chromatin immunoprecipitation microarray (ChIP-chip) to examine acetyl-H3K9 and dimethyl-H3K9. We found that the relative level of acetyl-H3K9 at different chromatin positions has a wider range of distribution than that of dimethyl-H3K9. We then used differential methylation hybridization (DMH) and the restriction landmark genome scanning (RLGS) to analyze the DNA methylation status of the same targets investigated by ChIP-chip. The results of epigenomic profiling, which have been independently confirmed for individual loci, show an inverse relationship between DNA methylation and histone acetylation in regulating gene silencing. In contrast to the previous notion, dimethyl-H3K9 seems to be less distinct in specifying silencing for the genes tested.
conclusionThis study demonstrates in L1210 leukemia cells a diverse relationship between histone modifications and DNA methylation in the maintenance of gene silencing. Acetyl-H3K9 shows an inverse relationship between DNA methylation and histone acetylation in regulating gene silencing as expected. However, dimethyl-H3K9 seems to be less distinct in relation to promoter methylation. Meanwhile, a combination of epigenomic tools is of help in understanding the heterogeneity of epigenetic regulation, which may further our vision accumulated from single-gene studies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.