ArticleBMC microbiology2007
A novel nested multiplex polymerase chain reaction (PCR) assay for differential detection of Entamoeba histolytica, E. moshkovskii and E. dispar DNA in stool samples.
Article in BMC microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.
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Who cites it
75 citing papers in PubMed, 165 citations in OpenAlex.
- A Cross-Sectional Study ofBioMed research international · 2019Trial
- Exploration of Various Diagnostic Modalities for Detection of Amoebic Liver Abscess and Co-Occurrence of Other Infective Aetiology, Eastern India.Tropical medicine & international health : TM & IH · 2026Observational
- First Detection and Molecular Characterization of Entamoeba spp. in Pet Mongolian Gerbils (Meriones unguiculatus) and Fat-Tailed Gerbils (Pachyuromys duprasi) in China.Acta parasitologica · 2026Article
- Gastrointestinal Parasites in Humans and Rhesus Macaques: A Cross-Sectional Study in Bhaktapur, Nepal.Health science reports · 2025Article
- Genotypic characterization ofFood and waterborne parasitology · 2025Article
- Moderate physical activity during late pregnancy enhances gut microbial network stability in pregnant women.Frontiers in microbiology · 2025Article
- Article
- Genetic characterization of theParasitology · 2024Article
- Amebic liver abscess: An update.World journal of hepatology · 2024Review
- Development of nucleic acid lateral flow immunoassay for molecular detection of Entamoeba moshkovskii and Entamoeba dispar in stool samples.Scientific reports · 2024Article
- Molecular Detection of a PathogenicPolish journal of microbiology · 2024Article
- Creep in nitroimidazole inhibitory concentration among the Entamoeba histolytica isolates causing amoebic liver abscess and screening of andrographolide as a repurposing drug.Scientific reports · 2023Article
- Amoebiasis: Advances in Diagnosis, Treatment, Immunology Features and the Interaction with the Intestinal Ecosystem.International journal of molecular sciences · 2023Review
- Prevalence and molecular characterization of Entamoeba moshkovskii in diarrheal patients from Eastern India.PLoS neglected tropical diseases · 2023Article
- Frequency ofJournal of parasitology research · 2023Article
- Molecular diagnosis of intestinal protozoa in young adults and their pets in Colombia, South America.PloS one · 2023Article
- A Comparative Analysis of Microscopy, Coproantigen Serology, and Nested Multiplex PCR in the Laboratory Diagnosis of Entamoeba histolytica Infection.Journal of laboratory physicians · 2022Article
- Molecular evidence suggests the occurrence of Entamoeba moshkovskii in pigs with zoonotic potential from eastern India.Folia parasitologica · 2022Article
- Detection ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2022Article
- Prevalence and Molecular Identification ofFrontiers in veterinary science · 2022Article
15 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundE. histolytica, a pathogenic amoeba, is indistinguishable in its cyst and trophozoite stages from those of non-pathogenic E. moshkovskii and E. dispar by light microscopy. We have developed a nested multiplex PCR targeting a 16S-like rRNA gene for differential detection of all the three morphologically similar forms of E. histolytica, E. moshkovskii and E. dispar simultaneously in stool samples.
resultsThe species specific product size for E. histolytica, E. moshkovskii and E. dispar was 439, 553 and 174 bp respectively, which was clearly different for all the three Entamoeba species. The nested multiplex PCR showed a sensitivity of 94% and specificity of 100% for the demonstration of E. histolytica, E. moshkovskii and E. dispar DNA in stool samples. The PCR was positive for E. histolytica, E. moshkovskii and E. dispar in a total of 190 out of 202 stool specimens (94% sensitive) that were positive for E. histolytica/E. dispar/E. moshkovskii by examination of stool by microscopy and/or culture. All the 35 negative control stool samples that were negative for E. histolytica/E. dispar/E. moshkovskii by microscopy and culture were also found negative by the nested multiplex PCR (100% specific). The result from the study shows that only 34.6% of the patient stool samples that were positive for E. histolytica/E. dispar/E. moshkovskii by examination of stool by microscopy and/or culture, were actually positive for pathogenic E. histolytica and the remaining majority of the stool samples were positive for non-pathogenic E. dispar or E. moshkovskii as demonstrated by the use of nested multiplex PCR.
conclusionThe present study reports a new nested multiplex PCR strategy for species specific detection and differentiation of E. histolytica, E. dispar and E. moshkovskii DNA in stool specimens. The test is highly specific, sensitive and also rapid, providing the results within 12 hours of receiving stool specimens.
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