ArticleMolecular biology of the cell2007
Interaction of the endocytic scaffold protein Pan1 with the type I myosins contributes to the late stages of endocytosis.
Article in Molecular biology of the cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 42 citations in OpenAlex.
- Cryptic genetic variation shapes the fate of gene duplicates in a protein interaction network.Nature communications · 2025Article
- Cryptic genetic variation shapes the fate of gene duplicates in a protein interaction network.bioRxiv : the preprint server for biology · 2024Article
- Actin- and microtubule-based motors contribute to clathrin-independent endocytosis in yeast.Molecular biology of the cell · 2023Article
- The protein architecture of the endocytic coat analyzed by FRET microscopy.Molecular systems biology · 2020Article
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- Article
- A Flow Cytometry-Based Phenotypic Screen To Identify Novel Endocytic Factors inG3 (Bethesda, Md.) · 2018Article
- High-speed superresolution imaging of the proteins in fission yeast clathrin-mediated endocytic actin patches.Molecular biology of the cell · 2018Article
- The TORC2-Dependent Signaling Network in the Yeast Saccharomyces cerevisiae.Biomolecules · 2017Review
- Article
- Polarized Exocytosis Induces Compensatory Endocytosis by Sec4p-Regulated Cortical Actin Polymerization.PLoS biology · 2016Article
- Actin-Regulator Feedback Interactions during Endocytosis.Biophysical journal · 2016Article
- A Pan1/End3/Sla1 complex links Arp2/3-mediated actin assembly to sites of clathrin-mediated endocytosis.Molecular biology of the cell · 2015Article
- Evolutionary rescue by compensatory mutations is constrained by genomic and environmental backgrounds.Molecular systems biology · 2015Article
- Pan1 regulates transitions between stages of clathrin-mediated endocytosis.Molecular biology of the cell · 2015Article
- Actin and endocytosis in budding yeast.Genetics · 2015Review
- Targeted disruption of an EH-domain protein endocytic complex, Pan1-End3.Traffic (Copenhagen, Denmark) · 2014Article
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- Role of Scd5, a protein phosphatase-1 targeting protein, in phosphoregulation of Sla1 during endocytosis.Journal of cell science · 2012Article
- Validation of regulated protein phosphorylation events in yeast by quantitative mass spectrometry analysis of purified proteins.Proteomics · 2012Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The yeast endocytic scaffold Pan1 contains an uncharacterized proline-rich domain (PRD) at its carboxy (C)-terminus. We report that the pan1-20 temperature-sensitive allele has a disrupted PRD due to a frame-shift mutation in the open reading frame of the domain. To reveal redundantly masked functions of the PRD, synthetic genetic array screens with a pan1DeltaPRD strain found genetic interactions with alleles of ACT1, LAS17 and a deletion of SLA1. Through a yeast two-hybrid screen, the Src homology 3 domains of the type I myosins, Myo3 and Myo5, were identified as binding partners for the C-terminus of Pan1. In vitro and in vivo assays validated this interaction. The relative timing of recruitment of Pan1-green fluorescent protein (GFP) and Myo3/5-red fluorescent protein (RFP) at nascent endocytic sites was revealed by two-color real-time fluorescence microscopy; the type I myosins join Pan1 at cortical patches at a late stage of internalization, preceding the inward movement of Pan1 and its disassembly. In cells lacking the Pan1 PRD, we observed an increased lifetime of Myo5-GFP at the cortex. Finally, Pan1 PRD enhanced the actin polymerization activity of Myo5-Vrp1 complexes in vitro. We propose that Pan1 and the type I myosins interactions promote an actin activity important at a late stage in endocytic internalization.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.