ArticleAmerican journal of physiology. Cell physiology2007
Purinergic agonists stimulate lens Na-K-ATPase-mediated transport via a Src tyrosine kinase-dependent pathway.
Article in American journal of physiology. Cell physiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 33 citations in OpenAlex.
- GNature communications · 2022Article
- Activation of Piezo1 Increases Na,K-ATPase-Mediated Ion Transport in Mouse Lens.International journal of molecular sciences · 2022Article
- Ion Transport Regulation by TRPV4 and TRPV1 in Lens and Ciliary Epithelium.Frontiers in physiology · 2021Review
- ATP1A1 Integrates AKT and ERK Signaling via Potential Interaction With Src to Promote Growth and Survival in Glioma Stem Cells.Frontiers in oncology · 2019Article
- Correcting deregulated Fxyd1 expression rescues deficits in neuronal arborization and potassium homeostasis in MeCP2 deficient male mice.Brain research · 2018Article
- Src Family Kinase Links Insulin Signaling to Short Term Regulation of Na,K-ATPase in Nonpigmented Ciliary Epithelium.Journal of cellular physiology · 2017Article
- Upregulation of P2Y2 nucleotide receptor in human hepatocellular carcinoma cells.The Journal of international medical research · 2016Article
- The Significance of TRPV4 Channels and Hemichannels in the Lens and Ciliary Epithelium.Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics · 2016Review
- Damage to lens fiber cells causes TRPV4-dependent Src family kinase activation in the epithelium.Experimental eye research · 2015Article
- Purines in the eye: recent evidence for the physiological and pathological role of purines in the RPE, retinal neurons, astrocytes, Müller cells, lens, trabecular meshwork, cornea and lacrimal gland.Experimental eye research · 2014Review
- Nitric oxide regulation of Na, K-ATPase activity in ocular ciliary epithelium involves Src family kinase.Journal of cellular physiology · 2014Article
- Article
- DIDS inhibits Na-K-ATPase activity in porcine nonpigmented ciliary epithelial cells by a Src family kinase-dependent mechanism.American journal of physiology. Cell physiology · 2013Article
- DIDS and the Janus-faced Na⁺-K⁺-activated ATPase. Focus on "DIDS inhibits Na-K-ATPase activity in porcine nonpigmented ciliary epithelial cells by a Src family kinase-dependent mechanism".American journal of physiology. Cell physiology · 2013Article
- Purinergic activation of rat skeletal muscle membranes increases Vmax and Na+ affinity of the Na,K-ATPase and phosphorylates phospholemman and α1 subunits.Pflugers Archiv : European journal of physiology · 2012Article
- Extracellular ATP stimulates epithelial cell motility through Pyk2-mediated activation of the EGF receptor.Cellular signalling · 2011Article
- The effect of endothelin-1 on Src-family tyrosine kinases and Na,K-ATPase activity in porcine lens epithelium.Journal of cellular physiology · 2011Article
- Lens ion transport: from basic concepts to regulation of Na,K-ATPase activity.Experimental eye research · 2009Review
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3 authors at 1 institution in 1 country.
Funding
Abstract
The Na-K-ATPase is vital for maintenance of lens transparency. Past studies using intact lens suggested the involvement of tyrosine kinases in short-term regulation of Na-K-ATPase. Furthermore, in vitro phosphorylation of a lens epithelial membrane preparation by Src family kinases (SFKs), a family of nonreceptor tyrosine kinases, resulted in modification of Na-K-ATPase activity. Here, the effect of purinergic agonists, ATP and UTP, on Na-K-ATPase function and SFK activation was examined in the rabbit lens. Na-K-ATPase function was examined using two different approaches, measurement of ouabain-sensitive potassium ((86)Rb) uptake by the intact lens, and Na-K-ATPase activity in lens epithelial homogenates. ATP and UTP caused a significant increase in ouabain-sensitive potassium ((86)Rb) uptake. Na-K-ATPase activity was increased in the epithelium of lenses pretreated with ATP. Lenses treated with ATP or UTP displayed activation of SFKs as evidenced by increased Western blot band density of active SFK (phosphorylated at the active loop Y416) and decreased band density of inactive SFKs (phosphorylated at the COOH terminal). A single PY416-Src immunoreactive band at approximately 60 kDa was observed, suggesting not all Src family members are activated. Immunoprecipitation studies showed that band density of active Src, and to a lesser extent active Fyn, was significantly increased, while active Yes did not change. Preincubation of the lenses with SFK inhibitor PP2 abolished the ATP-induced increase in ouabain-sensitive potassium ((86)Rb) uptake. The results suggest selective activation of Src and/or Fyn is part of a signaling mechanism initiated by purinergic agonists that increases Na-K-ATPase-mediated transport in the organ-cultured lens.
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