ArticleTissue engineering2006
Structure and functional evaluation of tendon-skeletal muscle constructs engineered in vitro.
Article in Tissue engineering, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
58 citing papers in PubMed.
- Myotendinous Junction development and repair.Journal of orthopaedic translation · 2026Review
- Automated Microfluidics-Assisted Hydrogel-Based Wet-Spinning for the Biofabrication of Biomimetic Engineered Myotendinous Junction.Advanced healthcare materials · 2024Article
- Engineering interfacial tissues: The myotendinous junction.APL bioengineering · 2024Review
- An engineered in vitro model of the human myotendinous junction.Acta biomaterialia · 2024Article
- Tendon Extracellular Matrix Promotes Myotendinous Junction Protein Expression in Engineered Muscle Tissue under Both Static and Mechanically Stimulated Culture Conditions.Journal of tissue engineering and regenerative medicine · 2023Article
- A comprehensive review of computational and image analysis techniques for quantitative evaluation of striated muscle tissue architecture.Biophysics reviews · 2022Review
- A bioprinted complex tissue model for myotendinous junction with biochemical and biophysical cues.Bioengineering & translational medicine · 2022Article
- Skeletal muscle differentiation of human iPSCs meets bioengineering strategies: perspectives and challenges.NPJ Regenerative medicine · 2022Review
- Principles for the design of multicellular engineered living systems.APL bioengineering · 2022Article
- Available In Vitro Models for Human Satellite Cells from Skeletal Muscle.International journal of molecular sciences · 2021Review
- Engineering skeletal muscle: Building complexity to achieve functionality.Seminars in cell & developmental biology · 2021Review
- Regenerative medicine for skeletal muscle loss: a review of current tissue engineering approaches.Journal of materials science. Materials in medicine · 2021Review
- Tissue-Engineered Skeletal Muscle Models to Study Muscle Function, Plasticity, and Disease.Frontiers in physiology · 2021Review
- Bioinspired Scaffold Designs for Regenerating Musculoskeletal Tissue Interfaces.Regenerative engineering and translational medicine · 2020Article
- Adding exogenous biglycan or decorin improves tendon formation for equine peritenon and tendon proper cells in vitro.BMC musculoskeletal disorders · 2020Article
- Using tools in mechanobiology to repair tendons.Current tissue microenvironment reports · 2020Article
- Striated myocyte structural integrity: Automated analysis of sarcomeric z-discs.PLoS computational biology · 2020Article
- Innervation: the missing link for biofabricated tissues and organs.NPJ Regenerative medicine · 2020Review
- Engineered skeletal muscles for disease modeling and drug discovery.Biomaterials · 2019Review
- Biologics and stem cell-based therapies for rotator cuff repair.Annals of the New York Academy of Sciences · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
During muscle contraction, the integrity of the myotendinous junction (MTJ) is important for the transmission of force from muscle to tendon. We evaluated the contractile and structural characteristics of 3-dimensional (3-D) skeletal muscle constructs co-cultured with engineered self-organized tendon constructs (n = 4), or segments of adult (n = 4) or fetal (n = 5) rat-tail tendon. We hypothesized that the co-culture of tendon and muscle would produce constructs with viable muscle-tendon interfaces that remain intact during generation of force. Construct diameter (lm) and maximum isometric force (microN) were measured, and specific force (kPa) was determined. After measure of force, constructs were loaded at a constant strain rate until failure and surface strains were recorded optically across the tendon, the muscle and the interface and used to determine the tangent modulus (passive stiffness) of the construct. Frozen samples were used for Trichrome Masson staining and immunofluorescent analysis of the MTJ-specific protein paxillin. No differences were observed between the groups with respect to diameter, maximum force, or specific force. The MTJ was robust and withstood tensile loading beyond the physiological strain range. The majority of the constructs failed in the muscle region. At the MTJ, there is an increase in the expression and localization of paxillin. In conclusion, using 3 sources of tendon tissue, we successfully engineered 3-D muscle-tendon constructs with functionally viable MTJ, characterized by structural features and protein expression patterns resembling neonatal MTJs in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.