ArticleNucleic acids research2007
Tentacle probes: eliminating false positives without sacrificing sensitivity.
Article in Nucleic acids research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed.
- Stoichiometric approach to quantitative analysis of biomolecules: the case of nucleic acids.Analytical and bioanalytical chemistry · 2022Article
- Sequence-specific detection of different strains of LCMV in a single sample using tentacle probes.Virology journal · 2017Article
- A microchip electrophoresis-based fluorescence signal amplification strategy for highly sensitive detection of biomolecules.Chemical communications (Cambridge, England) · 2016Article
- Split Spinach Aptamer for Highly Selective Recognition of DNA and RNA at Ambient Temperatures.Chembiochem : a European journal of chemical biology · 2016Article
- Surpassing specificity limits of nucleic acid probes via cooperativity.The Journal of molecular diagnostics : JMD · 2010Article
- Fluorescent probes for live-cell RNA detection.Annual review of biomedical engineering · 2009Review
- Tentacle probe sandwich assay in porous polymer monolith improves specificity, sensitivity and kinetics.Nucleic acids research · 2008Article
- Interactive fluorophore and quencher pairs for labeling fluorescent nucleic acid hybridization probes.Molecular biotechnology · 2008Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The majority of efforts to increase specificity or sensitivity in biosensors result in trade-offs with little to no gain in overall accuracy. This is because a biosensor cannot be more accurate than the affinity interaction it is based on. Accordingly, we have developed a new class of reagents based on mathematical principles of cooperativity to enhance the accuracy of the affinity interaction. Tentacle probes (TPs) have a hairpin structure similar to molecular beacons (MBs) for enhanced specificity, but are modified by the addition of a capture probe for increased kinetics and affinity. They produce kinetic rate constants up to 200-fold faster than MB with corresponding stem strengths. Concentration-independent specificity was observed with no false positives at up to 1 mM concentrations of variant analyte. In contrast, MBs were concentration dependent and experienced false positives above 3.88 muM of variant analyte. The fast kinetics of this label-free reagent may prove important for extraction efficiency, hence sensitivity and detection time, in microfluidic assays. The concentration-independent specificity of TPs may prove extremely useful in assays where starting concentrations and purities are unknown as would be the case in bioterror or clinical point of care diagnostics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.