ArticleProceedings of the National Academy of Sciences of the United States of America2007
Multigene amplification and massively parallel sequencing for cancer mutation discovery.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.
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Who cites it
71 citing papers in PubMed.
- Target Enrichment Approaches for Next-Generation Sequencing Applications in Oncology.Diagnostics (Basel, Switzerland) · 2022Review
- Dealing with Pseudogenes in Molecular Diagnostics in the Next Generation Sequencing Era.Methods in molecular biology (Clifton, N.J.) · 2021Review
- Comprehensive assay for the molecular profiling of cancer by target enrichment from formalin-fixed paraffin-embedded specimens.Cancer science · 2019Article
- Detailed analysis of HTT repeat elements in human blood using targeted amplification-free long-read sequencing.Human mutation · 2018Article
- Nimbus: a design-driven analyses suite for amplicon-based NGS data.Bioinformatics (Oxford, England) · 2018Article
- Identification and characterization of TP53 gene Allele Dropout in Li-Fraumeni syndrome and Oral cancer cohorts.Scientific reports · 2018Article
- Limited reverse transcriptase activity of phi29 DNA polymerase.Nucleic acids research · 2018Article
- Sensitive and inexpensive digital DNA analysis by microfluidic enrichment of rolling circle amplified single-molecules.Nucleic acids research · 2017Article
- Targeted DNA sequencing and in situ mutation analysis using mobile phone microscopy.Nature communications · 2017Article
- Long-adapter single-strand oligonucleotide probes for the massively multiplexed cloning of kilobase genome regions.Nature biomedical engineering · 2017Article
- Article
- Clinical Versus Research Sequencing.Cold Spring Harbor perspectives in medicine · 2016Review
- A Synonymous Mutation Upstream of the Gene Encoding a Weak-Link Enzyme Causes an Ultrasensitive Response in Growth Rate.Journal of bacteriology · 2016Article
- Synonymous mutations in oncogenesis and apoptosis versus survival unveiled by network modeling.Oncotarget · 2016Article
- CNVkit: Genome-Wide Copy Number Detection and Visualization from Targeted DNA Sequencing.PLoS computational biology · 2016Article
- Clinical exome sequencing in neurogenetic and neuropsychiatric disorders.Annals of the New York Academy of Sciences · 2016Review
- Upfront molecular testing in patients with advanced gastro-esophageal cancer: Is it time yet?Oncotarget · 2015Article
- Mutation-induced protein interaction kinetics changes affect apoptotic network dynamic properties and facilitate oncogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- New lung cancer panel for high-throughput targeted resequencing.Genomics & informatics · 2014Article
- Next generation 1536-well oligonucleotide synthesizer with on-the-fly dispense.Journal of biotechnology · 2014Article
11 more citing papers are in PubMed but not listed here.
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Authors and funding
10 authors.
Funding
Abstract
We have developed a procedure for massively parallel resequencing of multiple human genes by combining a highly multiplexed and target-specific amplification process with a high-throughput parallel sequencing technology. The amplification process is based on oligonucleotide constructs, called selectors, that guide the circularization of specific DNA target regions. Subsequently, the circularized target sequences are amplified in multiplex and analyzed by using a highly parallel sequencing-by-synthesis technology. As a proof-of-concept study, we demonstrate parallel resequencing of 10 cancer genes covering 177 exons with average sequence coverage per sample of 93%. Seven cancer cell lines and one normal genomic DNA sample were studied with multiple mutations and polymorphisms identified among the 10 genes. Mutations and polymorphisms in the TP53 gene were confirmed by traditional sequencing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.