ArticleProceedings of the National Academy of Sciences of the United States of America2007
Early sorting of inner nuclear membrane proteins is conserved.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 40 citations in OpenAlex.
- Autographa californica multiple nucleopolyhedrovirusJournal of virology · 2024Article
- Lamin is essential for nuclear localization of the GPI synthesis enzyme PIG-B and GPI-anchored protein production inJournal of cell science · 2020Article
- Genomic profiling screens small molecules of metastatic prostate carcinoma.Oncology letters · 2015Article
- Live imaging and modeling of inner nuclear membrane targeting reveals its molecular requirements in mammalian cells.The Journal of cell biology · 2015Article
- Sorting nexin 6 enhances lamin a synthesis and incorporation into the nuclear envelope.PloS one · 2014Article
- Mapping of sequences in Pseudorabies virus pUL34 that are required for formation and function of the nuclear egress complex.Journal of virology · 2013Article
- Connecting the nucleus to the cytoskeleton by SUN-KASH bridges across the nuclear envelope.Current opinion in cell biology · 2013Review
- Many mechanisms, one entrance: membrane protein translocation into the nucleus.Cellular and molecular life sciences : CMLS · 2012Review
- Trafficking to uncharted territory of the nuclear envelope.Current opinion in cell biology · 2012Review
- Structural determinants for nuclear envelope localization and function of pseudorabies virus pUL34.Journal of virology · 2012Article
- Evolution: functional evolution of nuclear structure.The Journal of cell biology · 2011Review
- Multiple mechanisms actively target the SUN protein UNC-84 to the inner nuclear membrane.Molecular biology of the cell · 2011Article
- System analysis shows distinct mechanisms and common principles of nuclear envelope protein dynamics.The Journal of cell biology · 2011Article
- A classical NLS and the SUN domain contribute to the targeting of SUN2 to the inner nuclear membrane.The EMBO journal · 2010Article
- Baculovirus data suggest a common but multifaceted pathway for sorting proteins to the inner nuclear membrane.Journal of virology · 2009Article
- The lamin B receptor under transcriptional control of C/EBPepsilon is required for morphological but not functional maturation of neutrophils.Human molecular genetics · 2008Article
- Membrane-anchored growth factor, HB-EGF, on the cell surface targeted to the inner nuclear membrane.The Journal of cell biology · 2008Article
- Traversing the NPC along the pore membrane: targeting of membrane proteins to the INM.Nucleus (Austin, Tex.)Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Spodoptera frugiperda (Sf9) importin-alpha-16 is a translocon-associated protein that participates in the early sorting pathway of baculovirus integral membrane proteins destined for the inner nuclear membrane (INM). To discern whether sorting intermediate protein complexes like those observed in insect cells are also formed with mammalian INM proteins, cross-linked complexes of importin-alpha-16 with human lamin B receptor (LBR) and nurim were examined. Both LBR and nurim cross-link with Sf9 importin-alpha-16 during cotranslational membrane integration and remain proximal with importin-alpha-16 after integration into the endoplasmic reticulum membrane and release from the translocon. Human cells encode several isoforms of importin-alpha; to determine whether any of these isoforms may recognize INM-directed proteins, they were tested for their ability to cross-link with the viral-derived INM sorting motif sequence. One cross-linked adduct was detected with a 16-kDa isoform encoded from KPNA4 (KPNA-4-16). KPNA-4-16 was easily detected in microsomal membranes prepared from KPNA4-16 recombinant virus-infected cells and was also detected in microsomes prepared from HeLa cells. Together these observations suggest that elements of the early sorting pathway of INM-directed proteins mediated by importin-alpha-16 are highly conserved, and mammalian KPNA-4-16 is a candidate partner in sorting integral membrane proteins to the INM.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.