ArticleProceedings of the National Academy of Sciences of the United States of America2007
In situ measurement of the electrical potential across the phagosomal membrane using FRET and its contribution to the proton-motive force.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
45 citing papers in PubMed, 84 citations in OpenAlex.
- Acidic and alkaline conditions enhance Staphylococcus aureus α-toxin pore formation and cytotoxicity.PLoS pathogens · 2026Article
- Phosphoproteomic Profiling Reveals mTOR Signaling in Sustaining Macrophage Phagocytosis of Cancer Cells.Cancers · 2024Article
- Two-pore channels regulate endomembrane tension to enable remodeling and resolution of phagolysosomes.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Efferocytosis requires periphagosomal CaNature communications · 2022Article
- Current methods to analyze lysosome morphology, positioning, motility and function.Traffic (Copenhagen, Denmark) · 2022Review
- Phagosomal chloride dynamics in the alveolar macrophage.iScience · 2022Article
- Imaging the electrical activity of organelles in living cells.Communications biology · 2021Article
- Sodium channels enable fast electrical signaling and regulate phagocytosis in the retinal pigment epithelium.BMC biology · 2019Article
- Candida albicans Hyphal Expansion Causes Phagosomal Membrane Damage and Luminal Alkalinization.mBio · 2018Article
- Membrane potential and dynamics in a ternary lipid mixture: insights from molecular dynamics simulations.Physical chemistry chemical physics : PCCP · 2018Article
- Bunyavirus requirement for endosomal K+ reveals new roles of cellular ion channels during infection.PLoS pathogens · 2018Article
- Role of the phagosomal redox-sensitive TRP channel TRPM2 in regulating bactericidal activity of macrophages.Journal of cell science · 2017Article
- Acid-inducible proton influx currents in the plasma membrane of murine osteoclast-like cells.Pflugers Archiv : European journal of physiology · 2016Article
- H-Rubies, a new family of red emitting fluorescent pH sensors for living cells.Chemical science · 2015Article
- Salt, chloride, bleach, and innate host defense.Journal of leukocyte biology · 2015Review
- Article
- Molecular bioelectricity: how endogenous voltage potentials control cell behavior and instruct pattern regulation in vivo.Molecular biology of the cell · 2014Article
- Stepwise priming by acidic pH and a high K+ concentration is required for efficient uncoating of influenza A virus cores after penetration.Journal of virology · 2014Article
- The voltage-gated sodium channel TPC1 confers endolysosomal excitability.Nature chemical biology · 2014Article
- Autologous dendritic cells prolong allograft survival through Tmem176b-dependent antigen cross-presentation.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phagosomes employ lytic enzymes, cationic peptides, and reactive oxygen intermediates to eliminate invading microorganisms. The effectiveness of these microbicidal mechanisms is potentiated by the acidic pH created by H(+)-pumping vacuolar-type ATPases (V-ATPases) on the phagosomal membrane. The degree of phagosomal acidification varies greatly among neutrophils, macrophages, and dendritic cells and can be affected by diseases like cystic fibrosis. The determinants of phagosomal pH are not completely understood, but the permeability to ions that neutralize the electrogenic effect of the V-ATPase has been proposed to play a central role. When counterion conductance is limiting, generation of a large membrane potential will dominate the proton-motive force (pmf), with a proportionally diminished pH gradient. Validation of this notion requires direct measurement of the electrical potential that develops across the phagosomal membrane (Psi(Phi)). We describe a noninvasive procedure to estimate Psi(Phi) in intact cells, based on fluorescence resonance energy transfer. This approach, in combination with measurements of phagosomal pH, enabled us to calculate the pmf across phagosomes of murine macrophages and to analyze the factors that limit acidification. At steady state, Psi(Phi) averaged 27 mV (lumen positive) and was only partially dissipated by inhibition of the V-ATPase with concanamycin A. The comparatively small contribution of the potential to the pmf suggests that proton pumping is not limited by the counterion permeability, a notion that was validated independently by using ionophores. Instead, phagosomal pH stabilizes when the rate of proton pumping, which decreases gradually as the lumen acidifies, is matched by the passive leak of proton equivalents.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.