Evidence map›Paper›PMID 17514486›Full record

ArticleMarine biotechnology (New York, N.Y.)

Estimating the efficiency of fish cross-species cDNA microarray hybridization.

Raphael Cohen, Vered Chalifa-Caspi, Timothy D Williams, Meirav Auslander, Stephen G George, James K Chipman, Moshe Tom

Abstract read
PubMed Publisher
In one paragraph

Article in Marine biotechnology (New York, N.Y.). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
3.3field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 26 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. DNA microarrays for identifying fishes.Marine biotechnology (New York, N.Y.)
    Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 2 countries.

Raphael CohenNational Institute for Biotechnology in Negev, Ben Gurion University of Negev, Beer-Sheva 84105, Israel.
Vered Chalifa-Caspi
Timothy D Williams
Meirav Auslander
Stephen G George
James K Chipman
Moshe Tom
Ben-Gurion University of the Negev · ILIsrael Oceanographic and Limnological Research · ILUniversity of Birmingham · GBUniversity of Stirling · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Using an available cross-species cDNA microarray is advantageous for examining multigene expression patterns in non-model organisms, saving the need for construction of species-specific arrays. The aim of the present study was to estimate relative efficiency of cross-species hybridizations across bony fishes, using bioinformatics tools. The methodology may serve also as a model for similar evaluations in other taxa. The theoretical evaluation was done by substituting comparative whole-transcriptome sequence similarity information into the thermodynamic hybridization equation. Complementary DNA sequence assemblages of nine fish species belonging to common families or suborders and distributed across the bony fish taxonomic branch were selected for transcriptome-wise comparisons. Actual cross-species hybridizations among fish of different taxonomic distances were used to validate and eventually to calibrate the theoretically computed relative efficiencies.

Indexed as

AnimalsConserved SequenceEfficiencyFishesGene Expression ProfilingMaleNucleic Acid HybridizationOligonucleotide Array Sequence AnalysisSpecies Specificity

Identifiers

PMID17514486
OpenAlexW1966972328

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.