ArticleApplied and environmental microbiology2007
Suitability of flow cytometry for estimating bacterial biovolume in natural plankton samples: comparison with microscopy data.
Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Enhanced warming and bacterial biomass production as key factors for coastal hypoxia in the southwestern Baltic Sea.Scientific reports · 2024Article
- Implementation of Fluorescent-Protein-Based Quantification Analysis in L-Form Bacteria.Bioengineering (Basel, Switzerland) · 2024Article
- Cell Sorting-Directed Selection of Bacterial Cells in Bigger Sizes Analyzed by Imaging Flow Cytometry during Experimental Evolution.International journal of molecular sciences · 2023Article
- Article
- Computational Analysis of Microbial Flow Cytometry Data.mSystems · 2021Review
- Temperature Responses of Heterotrophic Bacteria in Co-culture With a Red SeaFrontiers in microbiology · 2021Article
- Article
- Single-cell microscopy of suspension cultures using a microfluidics-assisted cell screening platform.Nature protocols · 2018Article
- Dynamic cellular complexity of anoxygenic phototrophic sulfur bacteria in the chemocline of meromictic Lake Cadagno.PloS one · 2017Article
- Experimental Warming Decreases the Average Size and Nucleic Acid Content of Marine Bacterial Communities.Frontiers in microbiology · 2016Article
- More, smaller bacteria in response to ocean's warming?Proceedings. Biological sciences · 2015Article
- Fluorescence in situ hybridization and sequential catalyzed reporter deposition (2C-FISH) for the flow cytometric sorting of freshwater ultramicrobacteria.Frontiers in microbiology · 2015Article
- Flow cytometry total cell counts: a field study assessing microbiological water quality and growth in unchlorinated drinking water distribution systems.BioMed research international · 2013Article
- Metagenomics uncovers a new group of low GC and ultra-small marine Actinobacteria.Scientific reports · 2013Article
- Environmentally persistent free radicals amplify ultrafine particle mediated cellular oxidative stress and cytotoxicity.Particle and fibre toxicology · 2009Article
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Authors and funding
5 authors.
Funding
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Abstract
The relationship between flow cytometry data and epifluorescence microscopy measurements was assessed in bacterioplankton samples from 80 lakes to estimate bacterial biovolume and cell size distribution. The total counts of 4',6'-diamidino-2-phenylindole-stained cells estimated by both methods were significantly related, and the slope of their linear regression was not significantly different from 1, indicating that both methods produce very similar estimates of bacterial abundance. The relationships between side scatter (SSC) and 4',6'-diamidino-2-phenylindole fluorescence and cell volume (microscopy values) were improved by binning of the data in three frequency classes for each, but further increases in the number of classes did not improve these relationships. Side scatter was the best cell volume predictor, and significant relationships were observed between the SSC classes and the smallest (R2 = 0.545, P < 0.001, n = 80) and the largest (R2 = 0.544, P < 0.001, n = 80) microscopy bacterial-size classes. Based on these relationships, a reliable bacterial biomass estimation was obtained from the SSC frequency classes. Our study indicates that flow cytometry can be used to properly estimate bacterioplankton biovolume, with an accuracy similar to those of more time-consuming microscopy methods.
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