ArticlePlant physiology2007
Thioredoxin-linked proteins are reduced during germination of Medicago truncatula seeds.
Article in Plant physiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
51 citing papers in PubMed, 152 citations in OpenAlex.
- Redox-mediated regulation of ABSCISIC ACID-INSENSITIVE 5 affects seed germination and seedling development in Arabidopsis.Journal of experimental botany · 2026Article
- Linking redox and calcium signaling via thioredoxins.Journal of experimental botany · 2026Review
- Article
- Redox dynamics in seeds ofFrontiers in plant science · 2024Article
- Current Insights into the Redox Regulation Network in Plant Chloroplasts.Plant & cell physiology · 2023Review
- Exploring Redox Modulation of Plant UDP-Glucose Pyrophosphorylase.International journal of molecular sciences · 2023Article
- Redox Signaling in Plant Heat Stress Response.Antioxidants (Basel, Switzerland) · 2023Review
- Glucose-6-Phosphate Dehydrogenases: The Hidden Players of Plant Physiology.International journal of molecular sciences · 2022Review
- Article
- NewAntioxidants (Basel, Switzerland) · 2022Article
- GWAS and transcriptome analysis reveal MADS26 involved in seed germination ability in maize.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Genome-wide identification, characterization, evolution, and expression pattern analyses of the typical thioredoxin gene family in wheat (Frontiers in plant science · 2022Article
- A New Role for Plastid Thioredoxins in Seed Physiology in Relation to Hormone Regulation.International journal of molecular sciences · 2021Article
- Overexpression of thioredoxin m in chloroplasts alters carbon and nitrogen partitioning in tobacco.Journal of experimental botany · 2021Article
- Genome-Wide Association Studies Identifying Multiple Loci Associated With Alfalfa Forage Quality.Frontiers in plant science · 2021Article
- Involvement of the MetO/Msr System in TwoInternational journal of molecular sciences · 2020Article
- Biochemical insight into redox regulation of plastidial 3-phosphoglycerate dehydrogenase fromThe Journal of biological chemistry · 2020Article
- Redox-mediated kick-start of mitochondrial energy metabolism drives resource-efficient seed germination.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Thioredoxin Network in Plant Mitochondria: Cysteine S-Posttranslational Modifications and Stress Conditions.Frontiers in plant science · 2020Review
- The expression pattern of OsDim1 in rice and its proposed function.Scientific reports · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Germination of cereals is accompanied by extensive change in the redox state of seed proteins. Proteins present in oxidized form in dry seeds are converted to the reduced state following imbibition. Thioredoxin (Trx) appears to play a role in this transition in cereals. It is not known, however, whether Trx-linked redox changes are restricted to cereals or whether they take place more broadly in germinating seeds. To gain information on this point, we have investigated a model legume, Medicago truncatula. Two complementary gel-based proteomic approaches were followed to identify Trx targets in seeds: Proteins were (1) labeled with a thiol-specific probe, monobromobimane (mBBr), following in vitro reduction by an NADP/Trx system, or (2) isolated on a mutant Trx affinity column. Altogether, 111 Trx-linked proteins were identified with few differences between axes and cotyledons. Fifty nine were new, 34 found previously in cereal or peanut seeds, and 18 in other plants or photosynthetic organisms. In parallel, the redox state of proteins assessed in germinating seeds using mBBr revealed that a substantial number of proteins that are oxidized or partly reduced in dry seeds became more reduced upon germination. The patterns were similar for proteins reduced in vivo during germination or in vitro by Trx. In contrast, glutathione and glutaredoxin were less effective as reductants in vitro. Overall, more than half of the potential targets identified with the mBBr labeling procedure were reduced during germination. The results provide evidence that Trx functions in the germination of seeds of dicotyledons as well as monocotyledons.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.