ArticleJournal of bacteriology2007
PdhR (pyruvate dehydrogenase complex regulator) controls the respiratory electron transport system in Escherichia coli.
Article in Journal of bacteriology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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Who cites it
66 citing papers in PubMed, 147 citations in OpenAlex.
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- Sensitivities in protein allocation models reveal distribution of metabolic capacity and flux control.Bioinformatics (Oxford, England) · 2024Article
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- A master regulator of central carbon metabolism directly activates virulence gene expression in attaching and effacing pathogens.PLoS pathogens · 2024Article
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- Enhancing Escherichia coli abiotic stress resistance through ornithine lipid formation.Applied microbiology and biotechnology · 2024Article
- Allosteric-Regulation-Based DNA Circuits in Saccharomyces cerevisiae to Detect Organic Acids and Monitor Hydrocarbon Metabolism In Vitro.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Transcriptome Analysis Reveals the Effect of PdhR inInternational journal of molecular sciences · 2023Article
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- OptimizingFrontiers in bioengineering and biotechnology · 2023Article
- Transcriptomics combined with metabolomics unveiled the key genes and metabolites of mycelium growth inFrontiers in microbiology · 2023Article
- Comparison of the genetic basis of biofilm formation betweenMicrobial genomics · 2022Article
- Is energy excess the initial trigger of carbon overflow metabolism? Transcriptional network response of carbon-limited Escherichia coli to transient carbon excess.Microbial cell factories · 2022Article
- Type I CRISPR-Cas provides robust immunity but incomplete attenuation of phage-induced cellular stress.Nucleic acids research · 2022Article
- Restoration of fitness lost due to dysregulation of the pyruvate dehydrogenase complex is triggered by ribosomal binding site modifications.Cell reports · 2021Article
6 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The pyruvate dehydrogenase (PDH) multienzyme complex plays a key role in the metabolic interconnection between glycolysis and the citric acid cycle. Transcription of the Escherichia coli genes for all three components of the PDH complex in the pdhR-aceEF-lpdA operon is repressed by the pyruvate-sensing PdhR, a GntR family transcription regulator, and derepressed by pyruvate. After a systematic search for the regulation targets of PdhR using genomic systematic evolution of ligands by exponential enrichment (SELEX), we have identified two novel targets, ndh, encoding NADH dehydrogenase II, and cyoABCDE, encoding the cytochrome bo-type oxidase, both together forming the pathway of respiratory electron transport downstream from the PDH cycle. PDH generates NADH, while Ndh and CyoABCDE together transport electrons from NADH to oxygen. Using gel shift and DNase I footprinting assays, the PdhR-binding site (PdhR box) was defined, which includes a palindromic consensus sequence, ATTGGTNNNACCAAT. The binding in vitro of PdhR to the PdhR box decreased in the presence of pyruvate. Promoter assays in vivo using a two-fluorescent-protein vector also indicated that the newly identified operons are repressed by PdhR and derepressed by the addition of pyruvate. Taken together, we propose that PdhR is a master regulator for controlling the formation of not only the PDH complex but also the respiratory electron transport system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.