ArticleBiophysical journal2007
Dynamics of firefly luciferase inhibition by general anesthetics: Gaussian and anisotropic network analyses.
Article in Biophysical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 43 citations in OpenAlex.
- Local Conformational Constraint of Firefly Luciferase Can Affect the Energy of Bioluminescence and Enzyme Stability.CCS chemistry · 2022Article
- General Anaesthesia Shifts the Murine Circadian Clock in a Time-Dependant Fashion.Clocks & sleep · 2021Article
- Gaussian network model can be enhanced by combining solvent accessibility in proteins.Scientific reports · 2017Article
- Identification of Hot Spots in Protein Structures Using Gaussian Network Model and Gaussian Naive Bayes.BioMed research international · 2016Article
- Article
- Sequence-based Gaussian network model for protein dynamics.Bioinformatics (Oxford, England) · 2014Article
- Seeing stem cells at work in vivo.Stem cell reviews and reports · 2014Review
- Article
- Multimodal imaging of stem cell implantation in the central nervous system of mice.Journal of visualized experiments : JoVE · 2012Article
- Inhibition of firefly luciferase by general anesthetics: effect on in vitro and in vivo bioluminescence imaging.PloS one · 2012Article
- Article
- Dynamical basis for drug resistance of HIV-1 protease.BMC structural biology · 2011Article
- The role of structured water in mediating general anesthetic action on alpha4beta2 nAChR.Physical chemistry chemical physics : PCCP · 2010Article
- Unresponsive correlated motion in alpha7 nAChR to halothane binding explains its functional insensitivity to volatile anesthetics.The journal of physical chemistry. B · 2010Article
- General anesthetic binding to neuronal alpha4beta2 nicotinic acetylcholine receptor and its effects on global dynamics.The journal of physical chemistry. B · 2009Article
- Asymmetric conformational flexibility in the ATP-binding cassette transporter HI1470/1.Biophysical journal · 2009Article
- Anesthetic modulation of protein dynamics: insight from an NMR study.The journal of physical chemistry. B · 2008Article
- A hypothesis on the origin and evolution of the response to inhaled anesthetics.Anesthesia and analgesia · 2008Review
- Interaction of anesthetics with open and closed conformations of a potassium channel studied via molecular dynamics and normal mode analysis.Biophysical journal · 2008Article
- Four-alpha-helix bundle with designed anesthetic binding pockets. Part I: structural and dynamical analyses.Biophysical journal · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The new crystal structures of the product-bound firefly luciferase combined with the previously determined substrate-free structures allow for a detailed analysis of the dynamics basis for the luciferase enzymatic activities. Using the Gaussian network model and the anisotropic network model, we show here that the superposition of the three slowest anisotropic network model modes, consisting of the bending, rotating, and rocking motions of the C-domain, accounts for large rearrangement of domains from the substrate-free (open) to product-bound (closed) conformation and thus constitutes a critical component of the enzyme's functions. The analysis also offers a unique platform to reexamine the molecular mechanism of the anesthetic inhibition of the firefly luciferase. Through perturbing the protein backbone network by introducing additional nodes to represent anesthetics, we found that the presence of two representative anesthetics, halothane and n-decanol, in different regions of luciferase had distinctively different effects on the protein's global motion. Only at the interface of the C- and N-domains did the anesthetics cause the most profound reduction in the overall flexibility of the C-domain and the concomitant increase in the flexibility of the loop, where the substitution of a conserved lysine residue was found experimentally to lead to >2-3 orders of magnitude reduction in activity. These anesthetic-induced dynamics changes can alter the normal function of the protein, appearing as an epiphenomenon of an "inhibition". The implication of the study is that a leading element for general anesthetic action on proteins is to disrupt the modes of motion essential to protein functions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.