ArticleBiochemical and biophysical research communications2007
Establishment of three-dimensional cultures of human pancreatic duct epithelial cells.
Article in Biochemical and biophysical research communications, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 39 citations in OpenAlex.
- DNA origami assembled spheroid for evaluating cytotoxicity and infiltration of chimeric antigen receptor macrophage (CAR-M).Communications biology · 2024Article
- Overview of carboxyl‑terminal modulator protein 1 and its importance in various metabolic regulations (Review).Molecular medicine reports · 2024Review
- Modulation of Epithelial-Mesenchymal Transition Is a Possible Underlying Mechanism for Inducing Chemoresistance in MIA PaCa-2 Cells against Gemcitabine and Paclitaxel.Biomedicines · 2024Article
- Mitoxantrone and Mitoxantrone-Loaded Iron Oxide Nanoparticles Induce Cell Death in Human Pancreatic Ductal Adenocarcinoma Cell Spheroids.Materials (Basel, Switzerland) · 2023Article
- EUS-FNA Biopsies to Guide Precision Medicine in Pancreatic Cancer: Results of a Pilot Study to IdentifyFrontiers in oncology · 2021Article
- Review
- The A818-6 system as an in-vitro model for studying the role of the transportome in pancreatic cancer.BMC cancer · 2020Article
- Article
- Engineered materials to model human intestinal development and cancer using organoids.Experimental cell research · 2019Review
- Designer hydrogels: Shedding light on the physical chemistry of the pancreatic cancer microenvironment.Cancer letters · 2018Review
- Modeling pancreatic cancer with organoids.Trends in cancer · 2016Article
- Pancreatic cancer organotypics: High throughput, preclinical models for pharmacological agent evaluation.World journal of gastroenterology · 2014Review
- Identification and differentiation of PDX1 β-cell progenitors within the human pancreatic epithelium.World journal of diabetes · 2014Article
- Single primary fetal lung cells generate alveolar structures in vitro.In vitro cellular & developmental biology. Animal · 2014Article
- From bench to bedside: lessons learned in translating preclinical studies in cancer drug development.Journal of the National Cancer Institute · 2013Review
- 3D pancreatic carcinoma spheroids induce a matrix-rich, chemoresistant phenotype offering a better model for drug testing.BMC cancer · 2013Article
- Activated Ras protein accelerates cell cycle progression to perturb Madin-Darby canine kidney cystogenesis.The Journal of biological chemistry · 2012Article
- A novel 3-dimensional culture system uncovers growth stimulatory actions by TGFβ in pancreatic cancer cells.Cancer biology & therapy · 2011Article
- Mn bioavailability by polarized Caco-2 cells: comparison between Mn gluconate and Mn oxyprolinate.Nutrition journal · 2011Article
- Consensus report of the national cancer institute clinical trials planning meeting on pancreas cancer treatment.Journal of clinical oncology : official journal of the American Society of Clinical Oncology · 2009Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Three-dimensional (3D) cultures of epithelial cells offer singular advantages for studies of morphogenesis or the role of cancer genes in oncogenesis. In this study, as part of establishing a 3D culture system of pancreatic duct epithelial cells, we compared human pancreatic duct epithelial cells (HPDE-E6E7) with pancreatic cancer cell lines. Our results show, that in contrast to cancer cells, HPDE-E6E7 organized into spheroids with what appeared to be apical and basal membranes and a luminal space. Immunostaining experiments indicated that protein kinase Akt was phosphorylated (Ser473) and CTMP, a negative Akt regulator, was expressed in both HPDE-E6E7 and cancer cells. However, a nuclear pool of CTMP was detectable in HPDE-E6E7 cells that showed a dynamic concentrated expression pattern, a feature that further distinguished HPDE-E637 cells from cancer cells. Collectively, these data suggest that 3D cultures of HPDE-E6E7 cells are useful for investigating signaling and morphological abnormalities in pancreatic cancer cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.