ArticleBMC biotechnology2007
Comparison of target labeling methods for use with Affymetrix GeneChips.
Article in BMC biotechnology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed.
- The Microglial Transcriptome of Age-Associated Deep Subcortical White Matter Lesions Suggests a Neuroprotective Response to Blood-Brain Barrier Dysfunction.International journal of molecular sciences · 2024Article
- Influence of RNA labeling on expression profiling of microRNAs.The Journal of molecular diagnostics : JMD · 2012Article
- Gene expression profiling at early organogenesis reveals both common and diverse mechanisms in foregut patterning.Developmental biology · 2011Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSeveral different commercial one-cycle labeling kits are available for preparation of the target for use with the Affymetrix GeneChip platform. However, there have been no evaluations of these different kits to determine if comparable results were generated. We report on the cRNA target synthesis, labeling efficiency and hybridization results using the One-Cycle Target Labeling Assay (Affymetrix), the BioArray RNA Amplification and Labeling System (Enzo Life Sciences), and the Superscript RNA Amplification System (Invitrogen Life Technologies).
resultsThe only notable difference between kits was in the yield of cRNA target synthesized during in vitro transcription, where the BioArray assay had to be repeated several times in order to have sufficient target. However, each kit resulted in comparable signal and detection calls when hybridized to the Affymetrix GeneChip.
conclusionThese 3 one-cycle labeling kits produce comparable hybridization results. This provides users with several kit options and flexibility when using the Affymetrix system.
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