ArticlePLoS pathogens2007
Structure of GrlR and the implication of its EDED motif in mediating the regulation of type III secretion system in EHEC.
Article in PLoS pathogens, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- The small regulatory RNA DsrA silences the locus of enterocyte effacement of enteropathogenicmicroPublication biology · 2025Article
- Phenotypic diversity of type III secretion system activity in enteropathogenicJournal of medical microbiology · 2024Article
- The two sRNAs OmrA and OmrB indirectly repress transcription from the LEE1 promoter of enteropathogenic Escherichia coli.Folia microbiologica · 2023Article
- GrlR, a negative regulator in enteropathogenicFrontiers in microbiology · 2023Article
- TheProceedings of the National Academy of Sciences of the United States of America · 2020Article
- The Small Regulatory RNA Spot42 Inhibits Indole Biosynthesis to Negatively Regulate the Locus of Enterocyte Effacement of Enteropathogenic Escherichia coli.Microorganisms · 2017Article
- Hfq and three Hfq-dependent small regulatory RNAs-MgrR, RyhB and McaS-coregulate the locus of enterocyte effacement in enteropathogenic Escherichia coli.Pathogens and disease · 2017Article
- Lambda Red-mediated Recombineering in the Attaching and Effacing Pathogen Escherichia albertii.Biological procedures online · 2016Article
- Dynamics of expression and maturation of the type III secretion system of enteropathogenic Escherichia coli.Journal of bacteriology · 2014Article
- A distinct regulatory sequence is essential for the expression of a subset of nle genes in attaching and effacing Escherichia coli.Journal of bacteriology · 2012Article
- Expression and regulation of the Escherichia coli O157:H7 effector proteins NleH1 and NleH2.PloS one · 2012Article
- Coordinate control of the locus of enterocyte effacement and enterohemolysin genes by multiple common virulence regulators in enterohemorrhagic Escherichia coli.Infection and immunity · 2011Article
- Honing the message: post-transcriptional and post-translational control in attaching and effacing pathogens.Trends in microbiology · 2011Article
- Molecular characterization of GrlA, a specific positive regulator of ler expression in enteropathogenic Escherichia coli.Journal of bacteriology · 2010Article
- Transcriptional analysis of the grlRA virulence operon from Citrobacter rodentium.Journal of bacteriology · 2010Article
- Virulence regulation in Citrobacter rodentium: the art of timing.Microbial biotechnology · 2010Review
- The RNA binding protein CsrA is a pleiotropic regulator of the locus of enterocyte effacement pathogenicity island of enteropathogenic Escherichia coli.Infection and immunity · 2009Article
- Hfq affects the expression of the LEE pathogenicity island in enterohaemorrhagic Escherichia coli.Molecular microbiology · 2009Article
- Identification and characterization of the lipid-binding property of GrlR, a locus of enterocyte effacement regulator.The Biochemical journal · 2009Article
- A single aromatic residue in transcriptional repressor protein KorA is critical for cooperativity with its co-regulator KorB.Molecular microbiology · 2008Article
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Authors and funding
8 authors.
Funding
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Abstract
Enterohemorrhagic Escherichia coli (EHEC) is a common cause of severe hemorrhagic colitis. EHEC's virulence is dependent upon a type III secretion system (TTSS) encoded by 41 genes. These genes are organized in several operons clustered in the locus of enterocyte effacement. Most of the locus of enterocyte effacement genes, including grlA and grlR, are positively regulated by Ler, and Ler expression is positively and negatively modulated by GrlA and GrlR, respectively. However, the molecular basis for the GrlA and GrlR activity is still elusive. We have determined the crystal structure of GrlR at 1.9 A resolution. It consists of a typical beta-barrel fold with eight beta-strands containing an internal hydrophobic cavity and a plug-like loop on one side of the barrel. Strong hydrophobic interactions between the two beta-barrels maintain the dimeric architecture of GrlR. Furthermore, a unique surface-exposed EDED (Glu-Asp-Glu-Asp) motif is identified to be critical for GrlA-GrlR interaction and for the repressive activity of GrlR. This study contributes a novel molecular insight into the mechanism of GrlR function.
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