ArticleBMC cell biology2007
Phospholipase C and myosin light chain kinase inhibition define a common step in actin regulation during cytokinesis.
Article in BMC cell biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 66 citations in OpenAlex.
- Article
- PLCβ2 Promotes VEGF-Induced Vascular Permeability.Arteriosclerosis, thrombosis, and vascular biology · 2022Article
- Group BPathogens (Basel, Switzerland) · 2022Article
- The Drosophila anterior-posterior axis is polarized by asymmetric myosin activation.Current biology : CB · 2022Article
- Phospholipase C-related catalytically inactive protein regulates cytokinesis by protecting phosphatidylinositol 4,5-bisphosphate from metabolism in the cleavage furrow.Scientific reports · 2019Article
- Pharmacologic inhibition of phospholipase C in the brain attenuates early memory formation in the honeybee (Biology open · 2018Article
- Endoplasmic reticulum (ER) CaThe Journal of biological chemistry · 2017Article
- nsPEF-induced PIPBiochemistry and biophysics reports · 2017Article
- Mechanism of Axonal Contractility in Embryonic Drosophila Motor Neurons In Vivo.Biophysical journal · 2016Article
- Control of diverse subcellular processes by a single multi-functional lipid phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2].The Biochemical journal · 2016Review
- Calcium Signaling Is Required for Erythroid Enucleation.PloS one · 2016Article
- Calcium waves occur as Drosophila oocytes activate.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- PIP2: choreographer of actin-adaptor proteins in the HIV-1 dance.Trends in microbiology · 2014Review
- Salicylic acid modulates levels of phosphoinositide dependent-phospholipase C substrates and products to remodel the Arabidopsis suspension cell transcriptome.Frontiers in plant science · 2014Article
- Expression analysis of a stress-related phosphoinositide-specific phospholipase C gene in wheat (Triticum aestivum L.).PloS one · 2014Article
- Role of mTOR downstream effector signaling molecules in Francisella tularensis internalization by murine macrophages.PloS one · 2013Article
- The Arabidopsis DREB2 genetic pathway is constitutively repressed by basal phosphoinositide-dependent phospholipase C coupled to diacylglycerol kinase.Frontiers in plant science · 2013Article
- HIV Assembly and Budding: Ca(2+) Signaling and Non-ESCRT Proteins Set the Stage.Molecular biology international · 2012Article
- Calcium-sensing receptor modulates cell adhesion and migration via integrins.The Journal of biological chemistry · 2011Article
- Myosin light chain kinases and phosphatase in mitosis and cytokinesis.Archives of biochemistry and biophysics · 2011Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPhosphatidylinositol 4,5-bisphosphate (PIP2) is required for successful completion of cytokinesis. In addition, both PIP2 and phosphoinositide-specific phospholipase C (PLC) have been localized to the cleavage furrow of dividing mammalian cells. PLC hydrolyzes PIP2 to yield diacylglycerol (DAG) and inositol trisphosphate (IP3), which in turn induces calcium (Ca2+) release from the ER. Several studies suggest PIP2 must be hydrolyzed continuously for continued cleavage furrow ingression. The majority of these studies employ the N-substituted maleimide U73122 as an inhibitor of PLC. However, the specificity of U73122 is unclear, as its active group closely resembles the non-specific alkylating agent N-ethylmaleimide (NEM). In addition, the pathway by which PIP2 regulates cytokinesis remains to be elucidated.
resultsHere we compared the effects of U73122 and the structurally unrelated PLC inhibitor ET-18-OCH3 (edelfosine) on cytokinesis in crane-fly and Drosophila spermatocytes. Our data show that the effects of U73122 are indeed via PLC because U73122 and ET-18-OCH3 produced similar effects on cell morphology and actin cytoskeleton organization that were distinct from those caused by NEM. Furthermore, treatment with the myosin light chain kinase (MLCK) inhibitor ML-7 caused cleavage furrow regression and loss of both F-actin and phosphorylated myosin regulatory light chain from the contractile ring in a manner similar to treatment with U73122 and ET-18-OCH3.
conclusionWe have used multiple inhibitors to examine the roles of PLC and MLCK, a predicted downstream target of PLC regulation, in cytokinesis. Our results are consistent with a model in which PIP2 hydrolysis acts via Ca2+ to activate myosin via MLCK and thereby control actin dynamics during constriction of the contractile ring.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.