ArticlePlant cell reports2007
Combined expression of chitinase and lipid transfer protein genes in transgenic carrot plants enhances resistance to foliar fungal pathogens.
Article in Plant cell reports, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 115 citations in OpenAlex.
- Single-cell transcriptomic analyses reveal cellular and molecular patterns of rose petal responses to gray mold infection.Horticulture research · 2025Article
- Glucanases and Chitinases inMolecules (Basel, Switzerland) · 2024Article
- NGS transcriptomic analysis uncovers the possible resistance mechanisms of olive toFrontiers in plant science · 2023Article
- The potential of plant proteins as antifungal agents for agricultural applications.Synthetic and systems biotechnology · 2022Review
- Cytokinin drives assembly of the phyllosphere microbiome and promotes disease resistance through structural and chemical cues.The ISME journal · 2022Article
- Origin, evolution, breeding, and omics of Apiaceae: a family of vegetables and medicinal plants.Horticulture research · 2022Article
- Lipid Transfer Proteins (LTPs)-Structure, Diversity and Roles beyond Antimicrobial Activity.Antibiotics (Basel, Switzerland) · 2021Review
- Chitinase family genes in grape differentially expressed in a manner specific to fruit species in response to Botrytis cinerea.Molecular biology reports · 2020Article
- Gene Cloning, Expression, and Antifungal Activities of Permatin from Naked Oat (Avena nuda).Probiotics and antimicrobial proteins · 2019Article
- Article
- Expression analysis of chitinase upon challenge inoculation toBiotechnology reports (Amsterdam, Netherlands) · 2017Article
- Co-transformation of canola by chimeric chitinase and tlp genes towards improving resistance to Sclerotinia sclerotiorum.World journal of microbiology & biotechnology · 2016Article
- Oligonucleotide treatment causes flax β-glucanase up-regulation via changes in gene-body methylation.BMC plant biology · 2014Article
- Article
- A sweetpotato SRD1 promoter confers strong root-, taproot-, and tuber-specific expression in Arabidopsis, carrot, and potato.Transgenic research · 2012Article
- Localization of DIR1 at the tissue, cellular and subcellular levels during Systemic Acquired Resistance in Arabidopsis using DIR1:GUS and DIR1:EGFP reporters.BMC plant biology · 2011Article
- High-level expression of human interferon alpha-2b in transgenic carrot (Daucus carota L.) plants.Plant cell reports · 2011Article
- The tomato xylem sap protein XSP10 is required for full susceptibility to Fusarium wilt disease.Journal of experimental botany · 2011Article
- Impact of endochitinase-transformed white spruce on soil fungal biomass and ectendomycorrhizal symbiosis.Applied and environmental microbiology · 2010Article
- Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two pathogenesis-related (PR) protein genes consisting of a barley chitinase (chi-2) and a wheat lipid-transfer-protein (ltp) were introduced singly and in combination into carrot plants via Agrobacterium-mediated transformation using the phosphinothricin acetyl transferase (bar) gene as a selectable marker. Over 75% of regenerated plants were confirmed to be positive for the transgenes by PCR and RT-PCR and were resistant to the herbicide Liberty (0.2%, v/v). Northern analysis and immunoblotting confirmed the expression of the transgenes in about 70% of the plants, with variable expression levels among individual lines. Southern analysis revealed from one to three copies of each transgene. Transgenic plants were inoculated with two necrotrophic foliar fungal pathogens, Alternaria radicicola and Botrytis cinerea, and showed significantly higher resistance when both PR genes were expressed compared to single-gene transformants. The level of disease reduction in plants expressing both genes was 95% for Botrytis and 90% for Alternaria infection compared to 40-50% for single-gene transformants. The chi2 and ltp genes could be deployed in combination in other crop plants to significantly enhance resistance to necrotrophic fungal pathogens.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.