ArticleMolecular biology of the cell2007
Spc24 and Stu2 promote spindle integrity when DNA replication is stalled.
Article in Molecular biology of the cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 26 citations in OpenAlex.
- A coordinated kinase and phosphatase network regulates Stu2 recruitment to yeast kinetochores.The Journal of cell biology · 2025Article
- Coupling DNA Replication and Spindle Function inCells · 2021Review
- Increased SPC24 in prostatic diseases and diagnostic value of SPC24 and its interacting partners in prostate cancer.Experimental and therapeutic medicine · 2021Article
- Cell-cycle phospho-regulation of the kinetochore.Current genetics · 2021Review
- Inhibition of spindle extension through the yeast S phase checkpoint is coupled to replication fork stability and the integrity of centromeric DNA.Molecular biology of the cell · 2019Article
- A Transcriptomic Insight into the Impact of Colon Cancer Cells on Mast Cells.International journal of molecular sciences · 2019Article
- The TOG protein Stu2/XMAP215 interacts covalently and noncovalently with SUMO.Cytoskeleton (Hoboken, N.J.) · 2018Article
- Molecular mechanisms facilitating the initial kinetochore encounter with spindle microtubules.The Journal of cell biology · 2017Article
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- Reverse PCA, a systematic approach for identifying genes important for the physical interaction between protein pairs.PLoS genetics · 2013Article
- Hsp110 is required for spindle length control.The Journal of cell biology · 2012Article
- Interactions between the kinetochore complex and the protein kinase A pathway in Saccharomyces cerevisiae.G3 (Bethesda, Md.) · 2012Article
- Ndc80 internal loop interacts with Dis1/TOG to ensure proper kinetochore-spindle attachment in fission yeast.Current biology : CB · 2011Article
- Laterally attached kinetochores recruit the checkpoint protein Bub1, but satisfy the spindle checkpoint.Cell cycle (Georgetown, Tex.) · 2010Article
- Stu1 inversely regulates kinetochore capture and spindle stability.Genes & development · 2009Article
- The coordination of centromere replication, spindle formation, and kinetochore-microtubule interaction in budding yeast.PLoS genetics · 2008Article
- Identification of CDCA8, DSN1 and BIRC5 in Regulating Cell Cycle and Apoptosis in Osteosarcoma Using Bioinformatics and Cell Biology.Technology in cancer research & treatmentArticle
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The kinetochore, a protein complex that links chromosomes to microtubules (MTs), is required to prevent spindle expansion during S phase in budding yeast, but the mechanism of how the kinetochore maintains integrity of the bipolar spindle before mitosis is not well understood. Here, we demonstrate that a mutation of Spc24, a component of the conserved Ndc80 kinetochore complex, causes lethality when cells are exposed to the DNA replication inhibitor hydroxyurea (HU) due to premature spindle expansion and segregation of incompletely replicated DNA. Overexpression of Stu1, a CLASP-related MT-associated protein or a truncated form of the XMAP215 orthologue Stu2 rescues spc24-9 HU lethality and prevents spindle expansion. Truncated Stu2 likely acts in a dominant-negative manner, because overexpression of full-length STU2 does not rescue spc24-9 HU lethality, and spindle expansion in spc24-9 HU-treated cells requires active Stu2. Stu1 and Stu2 localize to the kinetochore early in the cell cycle and Stu2 kinetochore localization depends on Spc24. We propose that mislocalization of Stu2 results in premature spindle expansion in S phase stalled spc24-9 mutants. Identifying factors that restrain spindle expansion upon inhibition of DNA replication is likely applicable to the mechanism by which spindle elongation is regulated during a normal cell cycle.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.