ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2007
Monitoring synaptic function at the neuromuscular junction of a mouse expressing synaptopHluorin.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- BACE1 Expression Is Required for Proper Synaptic Vesicle Dynamics in the Hippocampus.Journal of neurochemistry · 2025Article
- 25-Hydroxycholesterol modulates synaptic vesicle endocytosis at the mouse neuromuscular junction.Pflugers Archiv : European journal of physiology · 2025Article
- Postsynaptic Calcium Extrusion at the Mouse Neuromuscular Junction Alkalinizes the Synaptic Cleft.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- Spontaneously Recycling Synaptic Vesicles Constitute Readily Releasable Vesicles in Intact Neuromuscular Synapses.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2022Article
- "Calcium bombs" as harbingers of synaptic pathology and their mitigation by magnesium at murine neuromuscular junctions.Frontiers in molecular neuroscience · 2022Article
- Brain derived neurotrophic factor/tropomyosin related kinase B signaling impacts diaphragm neuromuscular transmission in a novel rat chemogenetic model.Frontiers in cellular neuroscience · 2022Article
- Presynaptic Mitochondria Communicate With Release Sites for Spatio-Temporal Regulation of Exocytosis at the Motor Nerve Terminal.Frontiers in synaptic neuroscience · 2022Article
- Calcium is reduced in presynaptic mitochondria of motor nerve terminals during neurotransmission in SMA mice.Human molecular genetics · 2021Article
- Zebrafish neuromuscular junction: The power of N.Neuroscience letters · 2019Review
- Presynaptic mechanisms controlling calcium-triggered transmitter release at the neuromuscular junction.Current opinion in physiology · 2018Article
- Impact of spatiotemporal calcium dynamics within presynaptic active zones on synaptic delay at the frog neuromuscular junction.Journal of neurophysiology · 2018Article
- Genetically encoded indicators of neuronal activity.Nature neuroscience · 2016Review
- The Active and Periactive Zone Organization and the Functional Properties of Small and Large Synapses.Frontiers in synaptic neuroscience · 2016Article
- Different dynamin blockers interfere with distinct phases of synaptic endocytosis during stimulation in motoneurones.The Journal of physiology · 2015Article
- Botulinum Neurotoxins Can Enter Cultured Neurons Independent of Synaptic Vesicle Recycling.PloS one · 2015Article
- Vesicular glutamate transporter expression level affects synaptic vesicle release probability at hippocampal synapses in culture.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Visualizing presynaptic function.Nature neuroscience · 2014Review
- Presynaptic long-term plasticity.Frontiers in synaptic neuroscience · 2013Review
- Segmentation of the mouse fourth deep lumbrical muscle connectome reveals concentric organisation of motor units.The Journal of physiology · 2013Article
- Organization and function of transmitter release sites at the neuromuscular junction.The Journal of physiology · 2013Review
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We monitored presynaptic exocytosis and vesicle recycling at neuromuscular junctions of transgenic mice expressing synaptopHluorin (spH), using simultaneous optical and electrophysiological recordings. Synaptic transmission was indistinguishable from that in wild-type controls. Fluorescence rose during and decayed monotonically after stimulus trains to the nerve, with amplitudes and decay times increasing with the amount of stimulation. The relatively large size of synaptic terminals allowed us to examine the spatial profile of fluorescence changes. We identified hot spots of exocytosis, which were stable with repeated trains. Photobleach experiments showed that spH freshly exposed by nerve stimulation was not preferentially retrieved by compensatory endocytosis; instead, most retrieved spH preexisted in the surface membrane. Finally, we compared fluorescence and electrical [summed end-plate potentials (EPPs)] estimates of exocytosis, which diverged during repeated trains, as fluorescence exceeded summed EPPs, although the average amplitude of miniature EPPs was unchanged. This might reflect exocytosis of spH-containing, acetylcholine-free ("empty") vesicles or other organelles during intense stimulation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.