ArticleJournal of clinical microbiology2007
Real-time PCR for determining capsular serotypes of Haemophilus influenzae.
Article in Journal of clinical microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
34 citing papers in PubMed.
- Trial
- Resurgence of invasive Haemophilus influenzae type b disease in France, 2017-2024: epidemiological, genomic, and immunological insights.NPJ vaccines · 2026Article
- Rapid and sensitive detection ofHeliyon · 2025Article
- Recurrent community-acquired pneumococcal meningitis in adults with and without identified predisposing factors.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Detection of Main Causative Agents among Young Children Suffering from Epiglottitis in Hilla City, Iraq.Archives of Razi Institute · 2023Article
- Epidemiology of invasiveInternational journal of circumpolar health · 2022Article
- Diagnosis of bacterial meningitis caused by Streptococcus pneumoniae, Neisseria meningitidis, and Haemophilus influenzae using a multiplex real-time PCR technique.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2022Article
- Impact of a Single-Tube PCR Assay for the Detection ofMicroorganisms · 2022Article
- Direct Real-Time PCR for the Detection and Serotyping of Haemophilus influenzae without DNA Extraction.Journal of clinical microbiology · 2022Article
- Evaluation of molecular typing for national surveillance of invasive clinicalFrontiers in microbiology · 2022Article
- The CINAMR (Clinical Information Network-Antimicrobial Resistance) Project: A pilot microbial surveillance using hospitals linked to regional laboratories in Kenya: Study Protocol.Wellcome open research · 2022Article
- The Role of Molecular Testing in Pediatric Meningitis Surveillance in Southern and East African Countries, 2008-2017.The Journal of infectious diseases · 2021Article
- The Burden of Invasive Bacterial Disease and the Impact of 10-Valent Pneumococcal Conjugate Vaccine in Children <5 years hospitalized for Meningitis in Lusaka, Zambia, 2010-2019.The Journal of infectious diseases · 2021Article
- Home Assessment of Indoor Microbiome (HAIM) in Relation to Lower Respiratory Tract Infections among Under-Five Children in Ibadan, Nigeria: The Study Protocol.International journal of environmental research and public health · 2020Article
- Genomic characterization of Haemophilus influenzae: a focus on the capsule locus.BMC genomics · 2019Article
- Performance of the Biomark HD real-time qPCR System (Fluidigm) for the detection of nasopharyngeal bacterial pathogens and Streptococcus pneumoniae typing.Scientific reports · 2019Article
- Nasopharyngeal carriage of Streptococcus pneumoniae, Haemophilus influenzae, and Staphylococcus aureus in a Brazilian elderly cohort.PloS one · 2019Observational
- Invasive Haemophilus influenzae Serotype a Infection in Children: Clinical Description of an Emerging Pathogen-Alaska, 2002-2014.The Pediatric infectious disease journal · 2018Article
- Single nucleotide polymorphisms in genes encoding penicillin-binding proteins in β-lactamase-negative ampicillin-resistant Haemophilus influenzae in Japan.BMC research notes · 2018Article
- Standardization of Laboratory Methods for the PERCH Study.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2017Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A two-step real-time PCR assay targeting all six capsulation loci of Haemophilus influenzae (i.e., serotypes a to f) was developed and compared with a previously published qualitative PCR assay by using 131 H. influenzae clinical isolates. There was a 98.5% concordance between the two tests. The sensitivity of detection of capsular type-specific reference strains of H. influenzae a to c (10(1) CFU/PCR) was higher than that for type e (10(3) CFU/PCR) and types d and f (10(4) CFU/PCR), and a broader dynamic range was obtained (5 to 8 log(10) units). No cross-reaction was observed with bacteria commonly isolated from the respiratory tract. We showed that both PCR assays are more reliable than slide agglutination serotyping. The real-time PCR-based assay seems to be an alternative of choice for the epidemiological follow-up of H. influenzae invasive infections.
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