ArticleJournal of virology2007
Minimal region sufficient for genome dimerization in the human immunodeficiency virus type 1 virion and its potential roles in the early stages of viral replication.
Article in Journal of virology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 40 citations in OpenAlex.
- Beyond reverse transcription: molecular mechanisms and emerging paradigms in retroviral replication.FEMS microbiology reviews · 2026Review
- Lentiviral Vectors: From Wild-Type Viruses to Efficient Multi-Functional Delivery Vectors.International journal of molecular sciences · 2025Review
- HIV-1 RNA genome packaging: it's G-rated.mBio · 2024Review
- Stability and conformation of the dimeric HIV-1 genomic RNA 5'UTR.Biophysical journal · 2021Article
- Unpaired Guanosines in the 5' Untranslated Region of HIV-1 RNA Act Synergistically To Mediate Genome Packaging.Journal of virology · 2020Article
- NMR Studies of Retroviral Genome Packaging.Viruses · 2020Review
- In cell mutational interference mapping experiment (in cell MIME) identifies the 5' polyadenylation signal as a dual regulator of HIV-1 genomic RNA production and packaging.Nucleic acids research · 2018Article
- Retroviral RNA Dimerization: From Structure to Functions.Frontiers in microbiology · 2018Review
- Interactions between HIV-1 Gag and Viral RNA Genome Enhance Virion Assembly.Journal of virology · 2017Article
- Transcriptional start site heterogeneity modulates the structure and function of the HIV-1 genome.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- SL1 revisited: functional analysis of the structure and conformation of HIV-1 genome RNA.Retrovirology · 2016Article
- On the Selective Packaging of Genomic RNA by HIV-1.Viruses · 2016Review
- Inhibition of HIV-1 reactivation by a telomerase-derived peptide in a HSP90-dependent manner.Scientific reports · 2016Article
- Conserved determinants of lentiviral genome dimerization.Retrovirology · 2015Article
- Mutational interference mapping experiment (MIME) for studying RNA structure and function.Nature methods · 2015Article
- Life of psi: how full-length HIV-1 RNAs become packaged genomes in the viral particles.Virology · 2014Review
- Annealing to sequences within the primer binding site loop promotes an HIV-1 RNA conformation favoring RNA dimerization and packaging.RNA (New York, N.Y.) · 2013Article
- A proposal for a new HIV-1 DLS structural model.Nucleic acids research · 2012Article
- Identification of a minimal region of the HIV-1 5'-leader required for RNA dimerization, NC binding, and packaging.Journal of molecular biology · 2012Article
- The determination of importance of sequences neighboring the Psi sequence in lentiviral vector transduction and packaging efficiency.PloS one · 2012Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It has been suggested that the dimer initiation site/dimer linkage sequence (DIS/DLS) region of the human immunodeficiency virus type 1 (HIV-1) RNA genome plays an important role at various stages of the viral life cycle. Recently we found that the duplication of the DIS/DLS region on viral RNA caused the production of partially monomeric RNAs in virions, indicating that this region indeed mediates RNA-RNA interaction. In this report, we followed up on this finding to identify the necessary and sufficient region for RNA dimerization in the virion of HIV-1. The region thus identified was 144 bases in length, extending from the junction of R/U5 and U5/L stem-loops to the end of SL4. The trans-acting responsive element, polyadenylation signal, primer binding site, upper stem-loop of U5/L, and SL2 were not needed for the function of this region. The insertion of this region into the ectopic location of the viral genome did not affect the level of virion production by transfection. However, the resultant virions contained monomerized genomes and showed drastic reductions in infectivity. A reduction was observed especially in the reverse transcription process. An attempt to generate a replication-competent virus with monomerized genome was performed by the long-term culture of mutant virus-infected cells. All recovered viruses were wild-type revertants, indicating a fatal defect of the mutation. These results suggest that genome dimerization or DIS/DLS itself also plays an important role in the early stages of virus infection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.