ArticleDevelopment (Cambridge, England)2007
Ena/VASP function in retinal axons is required for terminal arborization but not pathway navigation.
Article in Development (Cambridge, England), 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
39 citing papers in PubMed, 68 citations in OpenAlex.
- The Axonal Actin Filament Cytoskeleton: Structure, Function, and Relevance to Injury and Degeneration.Molecular neurobiology · 2024Review
- Filopodial protrusion driven by density-dependent Ena-TOCA-1 interactions.Journal of cell science · 2024Article
- Enabled primarily controls filopodial morphology, not actin organization, in the TSM1 growth cone inMolecular biology of the cell · 2023Article
- Antagonistic Activities of Fmn2 and ADF Regulate Axonal F-Actin Patch Dynamics and the Initiation of Collateral Branching.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2022Article
- Local mRNA translation and cytoskeletal reorganization: Mechanisms that tune neuronal responses.Frontiers in molecular neuroscience · 2022Review
- Cytoskeleton and Membrane Organization at Axon Branches.Frontiers in cell and developmental biology · 2021Review
- Axon growth and synaptic function: A balancing act for axonal regeneration and neuronal circuit formation in CNS trauma and disease.Developmental neurobiology · 2020Review
- A direct role for SNX9 in the biogenesis of filopodia.The Journal of cell biology · 2020Article
- Drosophila enabled promotes synapse morphogenesis and regulates active zone form and function.Neural development · 2020Article
- Filopodia and focal adhesions: An integrated system driving branching morphogenesis in neuronal pathfinding and angiogenesis.Developmental biology · 2019Review
- A Subtle Network Mediating Axon Guidance: Intrinsic Dynamic Structure of Growth Cone, Attractive and Repulsive Molecular Cues, and the Intermediate Role of Signaling Pathways.Neural plasticity · 2019Review
- Axon-Axon Interactions Regulate Topographic Optic Tract Sorting via CYFIP2-Dependent WAVE Complex Function.Neuron · 2018Article
- Filopodyan: An open-source pipeline for the analysis of filopodia.The Journal of cell biology · 2017Article
- It takes a village to raise a branch: Cellular mechanisms of the initiation of axon collateral branches.Molecular and cellular neurosciences · 2017Review
- A Requirement for Mena, an Actin Regulator, in Local mRNA Translation in Developing Neurons.Neuron · 2017Article
- Mutation of Kinesin-6 Kif20b causes defects in cortical neuron polarization and morphogenesis.Neural development · 2017Article
- Differential roles of α-, β-, and γ-actin in axon growth and collateral branch formation in motoneurons.The Journal of cell biology · 2017Article
- PCP Signaling between Migrating Neurons and their Planar-Polarized Neuroepithelial Environment Controls Filopodial Dynamics and Directional Migration.PLoS genetics · 2016Article
- Using Xenopus laevis retinal and spinal neurons to study mechanisms of axon guidance in vivo and in vitro.Seminars in cell & developmental biology · 2016Review
- Building Blocks of Functioning Brain: Cytoskeletal Dynamics in Neuronal Development.International review of cell and molecular biology · 2016Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
The Enabled/vasodilator-stimulated phosphoprotein (Ena/VASP) family of proteins is required for filopodia formation in growth cones and plays a crucial role in guidance cue-induced remodeling of the actin cytoskeleton. In vivo studies with pharmacological inhibitors of actin polymerization have previously provided evidence for the view that filopodia are needed for growth cone navigation in the developing visual pathway. Here we have re-examined this issue using an alternative strategy to generate growth cones without filopodia in vivo by artificially targeting Xena/XVASP (Xenopus homologs of Ena/VASP) proteins to mitochondria in retinal ganglion cells (RGCs). We used the specific binding of the EVH1 domain of the Ena/VASP family of proteins with the ligand motif FP4 to sequester the protein at the mitochondria surface. RGCs with reduced function of Xena/XVASP proteins extended fewer axons out of the eye and possessed dynamic lamellipodial growth cones missing filopodia that advanced slowly in the optic tract. Surprisingly, despite lacking filopodia, the axons navigated along the optic pathway without obvious guidance errors, indicating that the Xena/XVASP family of proteins and filopodial protrusions are non-essential for pathfinding in retinal axons. However, depletion of Xena/XVASP proteins severely impaired the ability of growth cones to form branches within the optic tectum, suggesting that this protein family, and probably filopodia, plays a key role in establishing terminal arborizations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.