ArticleGenome biology2007
A global view of gene expression in lithium and zinc treated sea urchin embryos: new components of gene regulatory networks.
Article in Genome biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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52 citing papers in PubMed, 92 citations in OpenAlex.
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- HES and Mox genes are expressed during early mesoderm formation in a mollusk with putative ancestral features.Scientific reports · 2021Article
- Neurotoxicity in Marine Invertebrates: An Update.Biology · 2021Review
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- A single cell RNA sequencing resource for early sea urchin development.Development (Cambridge, England) · 2020Article
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- Molecular patterning during the development ofEvoDevo · 2019Article
- Spatial and temporal patterns of gene expression during neurogenesis in the sea urchinEvoDevo · 2019Article
- Expression of six3 and otx in Solenogastres (Mollusca) supports an ancestral role in bilaterian anterior-posterior axis patterning.Evolution & development · 2018Article
- The evolutionary significance of polyploidy.Nature reviews. Genetics · 2017Review
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- Divergence of ectodermal and mesodermal gene regulatory network linkages in early development of sea urchins.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Development of the aboral domain in Nematostella requires β-catenin and the opposing activities of Six3/6 and Frizzled5/8.Development (Cambridge, England) · 2016Article
- Cooperative Wnt-Nodal Signals Regulate the Patterning of Anterior Neuroectoderm.PLoS genetics · 2016Article
- Neurogenic gene regulatory pathways in the sea urchin embryo.Development (Cambridge, England) · 2016Article
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Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe genome of the sea urchin Strongylocentrotus purpuratus has recently been sequenced because it is a major model system for the study of gene regulatory networks. Embryonic expression patterns for most genes are unknown, however.
resultsUsing large-scale screens on arrays carrying 50% to 70% of all genes, we identified novel territory-specific markers. Our strategy was based on computational selection of genes that are differentially expressed in lithium-treated embryos, which form excess endomesoderm, and in zinc-treated embryos, in which endomesoderm specification is blocked. Whole-mount in situ hybridization (WISH) analysis of 700 genes indicates that the apical organ region is eliminated in lithium-treated embryos. Conversely, apical and specifically neural markers are expressed more broadly in zinc-treated embryos, whereas endomesoderm signaling is severely reduced. Strikingly, the number of serotonergic neurons is amplified by at least tenfold in zinc-treated embryos. WISH analysis further indicates that there is crosstalk between the Wnt (wingless int), Notch, and fibroblast growth factor signaling pathways in secondary mesoderm cell specification and differentiation, similar to signaling cascades that function during development of presomitic mesoderm in mouse embryogenesis. We provide differential expression data for more than 4,000 genes and WISH patterns of more than 250 genes, and more than 2,400 annotated WISH images.
conclusionOur work provides tissue-specific expression patterns for a large fraction of the sea urchin genes that have not yet been included in existing regulatory networks and await functional integration. Furthermore, we noted neuron-inducing activity of zinc on embryonic development; this is the first observation of such activity in any organism.
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