ArticleBMC plant biology2007
Analysis of gene expression in cotton fiber initials.
Article in BMC plant biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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Who cites it
65 citing papers in PubMed, 141 citations in OpenAlex.
- Genome-wide identification, characterization and expression profiles of FORMIN gene family in cotton (Gossypium Raimondii L.).BMC genomic data · 2024Article
- An insight into the gene expression evolution in Gossypium species based on the leaf transcriptomes.BMC genomics · 2024Article
- Systematically and Comprehensively Understanding the Regulation of Cotton Fiber Initiation: A Review.Plants (Basel, Switzerland) · 2023Review
- A comprehensive overview of cotton genomics, biotechnology and molecular biological studies.Science China. Life sciences · 2023Review
- A deletion/duplication in the Ligon lintless-2 locus induces siRNAs that inhibit cotton fiber cell elongation.Plant physiology · 2022Article
- Data mining of transcriptional biomarkers at different cotton fiber developmental stages.Functional & integrative genomics · 2022Article
- Transcriptional Landscape of Cotton Fiber Development and Its Alliance With Fiber-Associated Traits.Frontiers in plant science · 2022Article
- Transcriptional loss of domestication-driven cytoskeletal GhPRF1 gene causes defective floral and fiber development in cotton (Gossypium).Plant molecular biology · 2021Article
- Comparative Metabolomics Analysis Reveals Sterols and Sphingolipids Play a Role in Cotton Fiber Cell Initiation.International journal of molecular sciences · 2021Article
- Mapping-by-sequencing the locus of EMS-induced mutation responsible for tufted-fuzzless seed phenotype in cotton.Molecular genetics and genomics : MGG · 2021Article
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- Elucidation of sequence polymorphism in fuzzless-seed cotton lines.Molecular genetics and genomics : MGG · 2021Article
- Genetic Analysis of the Transition from Wild to Domesticated Cotton (G3 (Bethesda, Md.) · 2020Article
- Expression Partitioning of Duplicate Genes at Single Cell Resolution inFrontiers in genetics · 2020Article
- Long non-coding RNAs and their potential functions in Ligon-lintless-1 mutant cotton during fiber development.BMC genomics · 2019Article
- Identification and profiling of upland cotton microRNAs at fiber initiation stage under exogenous IAA application.BMC genomics · 2019Article
- Genetic and transcriptomic dissection of the fiber length trait from a cotton (Gossypium hirsutum L.) MAGIC population.BMC genomics · 2019Article
- Interaction between calcium and potassium modulates elongation rate in cotton fiber cells.Journal of experimental botany · 2017Article
- Transcript profiling of genes expressed during fibre development in diploid cotton (Gossypium arboreum L.).BMC genomics · 2017Article
5 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCotton (Gossypium hirsutum L.) fibers are trichomes that initiate from the ovule epidermis. Little is known about the developmental pathway causing fiber to differentiate from ovular epidermal cells even though limits on the number of cells that differentiate into fiber will limit yield.
resultsA method was developed to isolate RNA from fiber initials 1 day post anthesis (dpa). Complementary DNA libraries representing 1 dpa fibers and other cotton tissues were sequenced and analyzed. Assembly of G. hirsutum Expressed Sequenced Tags (ESTs) identified over 11,000 sequences not previously represented in GenBank. New genes identified among these ESTs were represented on microarrays. The microarrays were used to identify genes enriched in fiber initials (1 dpa fibers) and elongating fibers. Analyses of Gene Ontologies (GO) of differentially expressed genes determined that terms associated with the "membranes" were statistically over represented among genes increased in expression in fiber initials and 10 dpa fibers. Staining ovules with a fluorescent dye confirmed an increase in Endoplasmic Reticulum (ER) occurred in fiber initials on the day of anthesis, persisted through 3 dpa and was absent in a fiberless mutant. Two genes similar to the CAPRICE/TRIPTYCHON (CPC) gene that inhibits differentiation of leaf trichomes in Arabidopsis were also characterized. Genes associated with novel regulation of brassinosterols, GTP mediated signal transduction and cell cycle control and components of a Ca+2 mediated signaling pathway were identified. Staining of cellular Ca+2 indicated that fiber initials had more Ca+2 than other ovule cells supporting a role for Ca+2 in fiber development.
conclusionAnalysis of genes expressed in fiber initials identified a unique stage in fiber development characterized by an increase in ER and Ca+2 levels that occurred between 0 and 1 dpa. The gene similar to CPC has a MYB domain but appears to lack a transcription activating domain similar to the Arabisopsis gene. The method used to stain the ER also can be used to count fiber initials and showed fiber cells develop from adjacent cells unlike leaf trichomes.
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