ArticleThe Biochemical journal2007
Novel xylan-binding properties of an engineered family 4 carbohydrate-binding module.
Article in The Biochemical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 32 citations in OpenAlex.
- Gatekeeping Dietary Fiber: The Role of Carbohydrate-Binding Modules in the Human Gut.Journal of microbiology and biotechnology · 2026Review
- Editor's Choice Protein engineering strategies to develop lectins by design.Glycobiology · 2025Review
- Directed Evolution of Material Binding Peptide for Polylactic Acid-specific Degradation in Mixed Plastic Wastes.ACS catalysis · 2023Article
- Strategies and Tactics for the Development of Selective Glycan-Binding Proteins.ACS chemical biology · 2021Review
- Replacement of carbohydrate binding modules improves acetyl xylan esterase activity and its synergistic hydrolysis of different substrates with xylanase.BMC biotechnology · 2016Article
- Crystallization, neutron data collection, initial structure refinement and analysis of a xyloglucan heptamer bound to an engineered carbohydrate-binding module from xylanase.Acta crystallographica. Section F, Structural biology communications · 2015Article
- Characterization of the substitution pattern of cellulose derivatives using carbohydrate-binding modules.BMC biotechnology · 2014Article
- Molecular and biochemical characterization of a new alkaline active multidomain xylanase from alkaline wastewater sludge.World journal of microbiology & biotechnology · 2013Article
- Uses of phage display in agriculture: a review of food-related protein-protein interactions discovered by biopanning over diverse baits.Computational and mathematical methods in medicine · 2013Review
- Versatile high resolution oligosaccharide microarrays for plant glycobiology and cell wall research.The Journal of biological chemistry · 2012Article
- Affinity maturation generates greatly improved xyloglucan-specific carbohydrate binding modules.BMC biotechnology · 2009Article
- Synthetic xylan-binding modules for mapping of pulp fibres and wood sections.BMC plant biology · 2007Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Molecular engineering of ligand-binding proteins is commonly used for identification of variants that display novel specificities. Using this approach to introduce novel specificities into CBMs (carbohydrate-binding modules) has not been extensively explored. Here, we report the engineering of a CBM, CBM4-2 from the Rhodothermus marinus xylanase Xyn10A, and the identification of the X-2 variant. As compared with the wild-type protein, this engineered module displays higher specificity for the polysaccharide xylan, and a lower preference for binding xylo-oligomers rather than binding the natural decorated polysaccharide. The mode of binding of X-2 differs from other xylan-specific CBMs in that it only has one aromatic residue in the binding site that can make hydrophobic interactions with the sugar rings of the ligand. The evolution of CBM4-2 has thus generated a xylan-binding module with different binding properties to those displayed by CBMs available in Nature.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.