Evidence map›Paper›PMID 17505812›Full record

ArticleClinical & experimental metastasis2007

MMP2 role in breast cancer brain metastasis development and its regulation by TIMP2 and ERK1/2.

Odete Mendes, Hun-Taek Kim, Gina Lungu, George Stoica

Abstract read
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In one paragraph

Article in Clinical & experimental metastasis, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 80 papers.

0numbers the graph read from it
0cells of the map it votes in
80citing papers in PubMed
3.5field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

80 citing papers in PubMed, 158 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Genomic and immune profiling of breast cancer brain metastases.Acta neuropathologica communications · 2025
    Article
  6. Review
  7. Article
  8. Review
  9. Review
  10. Article
  11. Article
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  14. The origin of brain malignancies at the blood-brain barrier.Cellular and molecular life sciences : CMLS · 2023
    Review
  15. Article
  16. Article
  17. Article
  18. Review
  19. Article
  20. Article

20 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Odete MendesDepartment of Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station, TX 77843, USA.
Hun-Taek Kim
Gina Lungu
George Stoica
Texas A&M University · US

Funding

Functional brain imaging by laser-induced PATR01NS046214 · NINDS · WASHINGTON UNIVERSITY · PI WANG, LIHONG · 2003 to 2007
$2.7M
NINDS NIH HHS R01-NS046214-01
6 · The paper itself

Abstract

Matrix metalloproteinase 2 (MMP2) is important in breast cancer (BC) invasion and metastasis. We previously reported that BC brain metastases, in a rat syngeneic model developed in our laboratory, have high expression and activity of MMP2. The MMP2 mechanism of action in the brain is still under intense scrutiny. To study the role of MMP2 in the development of BC brain metastasis we transfected ENU1564 rat mammary adenocarcinoma cells with tissue inhibitor of MMP2 (TIMP2). Animals inoculated with ENU1564-TIMP2 cells had decreased orthotopic tumor growth, decreased orthotopic metastatic behavior and did not develop brain metastases. These results were associated with decreased MMP2 activity, demonstrated by gel zymography. Mitogen activated protein kinase (MAPK) pathway components, such as ERK1/2, have been correlated to MMP expression and/or astrocyte activity. We found that BC brain metastases have peripheral astrocyte reactivity and higher expression of glial fibrillary acidic protein (GFAP) and phosphorylated-ERK1/2 (p-ERK1/2). Additionally, rat astrocyte-conditioned media increased in vitro invasion of ENU1564 cancer cells and increased expression of MMP2 and p-ERK1/2. Blockage of ERK1/2 phosphorylation by treatment with MEK inhibitor (PD98059) decreased the expression of MMP2 in cancer cells grown in rat astrocyte-conditioned media. Our results are highly suggestive that MMP2 plays a role in the development of BC metastases, in particular to the brain. Furthermore, our results suggest that astrocyte factors and the ERK1/2 signaling pathway may be associated with BC brain metastasis development; and that ERK1/2 may regulate MMP2 in a way that is modifiable by astrocyte factors.

Indexed as

AnimalsAstrocytesBrain NeoplasmsCulture Media, ConditionedDisease Models, AnimalFemaleFlavonoidsGlial Fibrillary Acidic ProteinMammary Neoplasms, ExperimentalMatrix Metalloproteinase 2Mitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Neoplasm InvasivenessPhosphorylationRatsSignal Transduction2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-oneCulture Media, ConditionedFlavonoidsGlial Fibrillary Acidic ProteinMatrix Metalloproteinase 2Mitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Tissue Inhibitor of Metalloproteinase-2

Identifiers

PMID17505812
OpenAlexW2038183997

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.