ArticleGenes & development2007
Environmental regulation operating at the promoter clearance step of bacterial transcription.
Article in Genes & development, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 39 citations in OpenAlex.
- Functional role of 18 LysR-Type transcriptional regulators of Salmonella enterica serovar Typhi.PloS one · 2025Article
- Characterization of the pleiotropic LysR-type transcription regulator LeuO of Escherichia coli.Nucleic acids research · 2019Article
- Diversity, versatility and complexity of bacterial gene regulation mechanisms: opportunities and drawbacks for applications in synthetic biology.FEMS microbiology reviews · 2019Review
- The transcriptional regulator LysG (Rv1985c) of Mycobacterium tuberculosis activates lysE (Rv1986) in a lysine-dependent manner.PloS one · 2017Article
- Engineering prokaryotic transcriptional activators as metabolite biosensors in yeast.Nature chemical biology · 2016Article
- Transcription profile of Escherichia coli: genomic SELEX search for regulatory targets of transcription factors.Nucleic acids research · 2016Article
- CstF-64 and 3'-UTR cis-element determine Star-PAP specificity for target mRNA selection by excluding PAPα.Nucleic acids research · 2016Article
- Distinct Paths for Basic Amino Acid Export in Escherichia coli: YbjE (LysO) Mediates Export of L-Lysine.Journal of bacteriology · 2015Article
- Metabolic engineering of Escherichia coli for enhanced arginine biosynthesis.Microbial cell factories · 2015Article
- Bordetella pertussis fim3 gene regulation by BvgA: phosphorylation controls the formation of inactive vs. active transcription complexes.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- The effect of cell growth phase on the regulatory cross-talk between flagellar and Spi1 virulence gene expression.PLoS pathogens · 2014Article
- Transcriptome analysis of Vibrio parahaemolyticus in type III secretion system 1 inducing conditions.Frontiers in cellular and infection microbiology · 2014Article
- Transcriptional cross-regulation between Gram-negative and gram-positive bacteria, demonstrated using ArgP-argO of Escherichia coli and LysG-lysE of Corynebacterium glutamicum.Journal of bacteriology · 2012Article
- Role of ArgP (IciA) in lysine-mediated repression in Escherichia coli.Journal of bacteriology · 2011Article
- Identification of ArgP and Lrp as transcriptional regulators of lysP, the gene encoding the specific lysine permease of Escherichia coli.Journal of bacteriology · 2011Article
- The poly A polymerase Star-PAP controls 3'-end cleavage by promoting CPSF interaction and specificity toward the pre-mRNA.The EMBO journal · 2010Article
- RNA polymerase trafficking in Bacillus subtilis cells.Journal of bacteriology · 2010Article
- Correct timing of dnaA transcription and initiation of DNA replication requires trans translation.Journal of bacteriology · 2009Article
- Regulator trafficking on bacterial transcription units in vivo.Molecular cell · 2009Article
- Lysine represses transcription of the Escherichia coli dapB gene by preventing its activation by the ArgP activator.Journal of bacteriology · 2008Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In vivo transcription of the Escherichia coli argO gene, which encodes an arginine (Arg) exporter, requires the LysR-family regulator protein ArgP (previously called IciA) and is induced in the presence of Arg or its naturally occurring antimetabolite analog canavanine. Lysine (Lys) addition, on the other hand, phenocopies an argP mutation to result in the shutoff of argO expression. We now report that the ArgP dimer by itself is able to bind the argO promoter-operator region to form a binary complex, but that the formation of a ternary complex with RNA polymerase is greatly stimulated only in presence of a coeffector. Both Arg and Lys were proficient as coeffectors for ArgP-mediated recruitment of RNA polymerase to, and open complex formation at, the argO promoter, although only Arg (but not Lys) was competent to activate transcription. The two coeffectors competed for binding to ArgP, and the ternary complex that had been assembled on the argO template in the presence of Lys could be chased into a transcriptionally active state upon Arg addition. Our results support a novel mechanism of argO regulation in which Lys-bound ArgP reversibly restrains RNA polymerase at the promoter, at a step (following open complex formation) that precedes, and is common to, both abortive and productive transcription. This represents, therefore, the first example of an environmental signal regulating the final step of promoter clearance by RNA polymerase in bacterial transcription. We propose that, in E. coli cells, the ternary complex remains assembled and poised at the argO promoter at all times to respond, positively or negatively, to instantaneous changes in the ratio of intracellular Arg to Lys concentrations.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.