ArticleBMC microbiology2007
Characterization of hypothetical proteins Cpn0146, 0147, 0284 & 0285 that are predicted to be in the Chlamydia pneumoniae inclusion membrane.
Article in BMC microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 23 citations in OpenAlex.
- A Functional Yeast-Based Screen Identifies the Host Microtubule Cytoskeleton as a Target of NumerousInternational journal of molecular sciences · 2023Article
- Chlamydia-induced curvature of the host-cell plasma membrane is required for infection.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Chlamydia pneumoniae inclusion membrane protein Cpn0147 interacts with host protein CREB3.PloS one · 2017Article
- Article
- Genome-wide identification of Chlamydia trachomatis antigens associated with trachomatous trichiasis.Investigative ophthalmology & visual science · 2012Article
- The transcriptional landscape of Chlamydia pneumoniae.Genome biology · 2011Article
- Genome-wide identification of Chlamydia trachomatis antigens associated with tubal factor infertility.Fertility and sterility · 2011Article
- Multi-genome identification and characterization of chlamydiae-specific type III secretion substrates: the Inc proteins.BMC genomics · 2011Article
- Chlamydia trachomatis secretion of proteases for manipulating host signaling pathways.Frontiers in microbiology · 2011Article
- Association of tubal factor infertility with elevated antibodies to Chlamydia trachomatis caseinolytic protease P.American journal of obstetrics and gynecology · 2010Article
- Serodiagnosis of Chlamydia pneumoniae infection using three inclusion membrane proteins.Journal of clinical laboratory analysis · 2010Article
- The chlamydial plasmid-encoded protein pgp3 is secreted into the cytosol of Chlamydia-infected cells.Infection and immunity · 2008Article
- Characterization of fifty putative inclusion membrane proteins encoded in the Chlamydia trachomatis genome.Infection and immunity · 2008Article
- Molecular mechanisms of host-pathogen interactions and their potential for the discovery of new drug targets.Current drug targets · 2008Review
- Rottlerin inhibits chlamydial intracellular growth and blocks chlamydial acquisition of sphingolipids from host cells.Applied and environmental microbiology · 2008Article
- Trafficking of chlamydial antigens to the endoplasmic reticulum of infected epithelial cells.Microbes and infectionArticle
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAlthough more than 100 Chlamydia pneumoniae hypothetical proteins have been predicted to be inclusion membrane proteins, only a few have been experimentally demonstrated to be in the inclusion membrane. Using antibodies raised with fusion proteins, we characterized four such hypothetical proteins encoded by two gene clusters (Cpn0146-147 and Cpn0284-285) in the C. pneumoniae genome.
resultsCpn0146 and 0147 were detected in the inclusion membrane while Cpn0284 and 0285 inside inclusion and mainly associated with reticulate bodies although all four proteins contain an N-terminal bi-lobed hydrophobic region, a signature motif assigned to inclusion membrane proteins. These four hypothetical proteins were only detected in cells infected with C. pneumoniae but not other chlamydial species, with Cpn0147 at 6 hours and Cpn0146, 0284 & 0285 at 24 hours after infection. Cpn0146 & 147 but not Cpn0284 and 285 co-localized with a host cell endoplasmic reticulum marker, a property known to be possessed by some chlamydial inclusion membrane proteins, when expressed in the host cell cytosol via transgenes. However, the endoplasmic reticulum localization of the C. pneumoniae inclusion membrane proteins did not result in inhibition of the subsequent C. pneumoniae infection.
conclusionThe hypothetical proteins Cpn0146 & 0147 were localized in the C. pneumoniae inclusion membrane while Cpn0284 & 0285 within the inclusion although all four were predicted to be Inc proteins, suggesting the need to experimentally characterize the predicted Inc proteins.
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