ArticlePlant cell reports2007
Isolation of two highly active soybean (Glycine max (L.) Merr.) promoters and their characterization using a new automated image collection and analysis system.
Article in Plant cell reports, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 64 citations in OpenAlex.
- Genomic and biochemical comparison of allelic triple-mutant lines derived from conventional breeding and multiplex gene editing.The plant genome · 2025Article
- Characterization of two constitutive promotersaBIOTECH · 2022Article
- Two efficient CRISPR/Cas9 systems for gene editing in soybean.Transgenic research · 2021Article
- Development and validation of a novel and robust cell culture system in soybean (Glycine max (L.) Merr.) for promoter screening.Plant cell reports · 2019Article
- Changes to the core and flanking sequences of G-box elements lead to increases and decreases in gene expression in both native and synthetic soybean promoters.Plant biotechnology journal · 2019Article
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- A novelBiotechnology for biofuels · 2017Article
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- A novel cis-acting element in the GmERF3 promoter contributes to inducible gene expression in soybean and tobacco after wounding.Plant cell reports · 2016Article
- Article
- Truncated Ubiquitin 5' Regulatory Region from Erianthus arundinaceus Drives Enhanced Transgene Expression in Heterologous Systems.Molecular biotechnology · 2015Article
- Development and optimization of agroinfiltration for soybean.Plant cell reports · 2015Article
- The intron and 5' distal region of the soybean Gmubi promoter contribute to very high levels of gene expression in transiently and stably transformed tissues.Plant cell reports · 2015Article
- Newly developed quantitative transactivation system shows difference in activation by Vitis CBF transcription factors on DRE/CRT elements.Plant methods · 2014Article
- Seed coat pigmentation in transgenic soybean expressing the silencing suppressor 2b gene of Cucumber mosaic virus.Plant cell reports · 2013Article
- A Novel Moderate Constitutive Promoter Derived from Poplar (Populus tomentosa Carrière).International journal of molecular sciences · 2013Article
- Enhanced transgene expression in sugarcane by co-expression of virus-encoded RNA silencing suppressors.PloS one · 2013Article
- Soybean genetic transformation: A valuable tool for the functional study of genes and the production of agronomically improved plants.Genetics and molecular biology · 2012Article
- Native polyubiquitin promoter of rice provides increased constitutive expression in stable transgenic rice plants.Plant cell reports · 2012Article
- Use of the VvMybA1 gene for non-destructive quantification of promoter activity via color histogram analysis in grapevine (Vitis vinifera) and tobacco.Transgenic research · 2011Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A novel automated image collection and analysis system was used to compare two new soybean (Glycine max (L.) Merr.) promoters with the cauliflower mosaic virus 35S (CaMV35S) promoter, which was used as an expression standard. For expression comparisons, various permutations of a soybean polyubiquitin (Gmubi) promoter, a soybean heat shock protein 90-like (GmHSP90L) promoter and the CaMV35S promoter were placed upstream of a green fluorescent protein (gfp) gene. DNA constructs were introduced via particle bombardment into excised cotyledons of germinating lima bean (Phaseolus lunatus L.) seeds, which were arranged in Petri dishes for automated image capture and image analysis. The automated system allowed monitoring and quantification of gfp gene expression in the same piece of tissue over time. The Gmubi promoter, with its intronic region intact, showed the highest expression that was over five times stronger than the CaMV35S promoter. When an intronic region was removed from the Gmubi promoter, GFP expression was reduced, but was still over two times greater than with the CaMV35S promoter. The full-length soybean GmHSP90L promoter was four times stronger than the CaMV35S promoter. Truncation of the GmHSP90L promoter resulted in stepwise decreases in promoter strength, which appear to correspond to removal of regulatory elements. Automated image capture and analysis allowed the rapid and efficient evaluation of these new promoters.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.