ArticleProceedings of the National Academy of Sciences of the United States of America2007
The frequency and structure of recombinant products is determined by the cellular level of MutL.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 45 citations in OpenAlex.
- Real-time monitoring of replication errors' fate reveals the origin and dynamics of spontaneous mutations.Nature communications · 2024Article
- Positive Selection during Niche Adaptation Results in Large-Scale and Irreversible Rearrangement of Chromosomal Gene Order in Bacteria.Molecular biology and evolution · 2022Article
- Speciation of Genes and Genomes: Conservation of DNA Polymorphism by Barriers to Recombination Raised by Mismatch Repair System.Frontiers in genetics · 2022Review
- Design and comparative characterization of RecA variants.Scientific reports · 2021Article
- Mismatch Repair: From Preserving Genome Stability to Enabling Mutation Studies in Real-Time Single Cells.Cells · 2021Review
- Competition for nutritional resources masks the true frequency of bacterial mutants.BMC biology · 2020Article
- Real-time visualization of mutations and their fitness effects in single bacteria.Nature protocols · 2019Article
- Mutation bias and GC content shape antimutator invasions.Nature communications · 2019Article
- Gene Expression Noise Produces Cell-to-Cell Heterogeneity in Eukaryotic Homologous Recombination Rate.Frontiers in genetics · 2019Article
- Article
- The poor homology stringency in the heteroduplex allows strand exchange to incorporate desirable mismatches without sacrificing recognition in vivo.Nucleic acids research · 2015Article
- Temperate phages acquire DNA from defective prophages by relaxed homologous recombination: the role of Rad52-like recombinases.PLoS genetics · 2014Article
- Mechanism of homologous recombination and implications for aging-related deletions in mitochondrial DNA.Microbiology and molecular biology reviews : MMBR · 2013Review
- Mismatch repair inhibits homeologous recombination via coordinated directional unwinding of trapped DNA structures.Molecular cell · 2013Article
- DNA Mismatch Repair.EcoSal Plus · 2012Article
- Involvement of mismatch repair in the reciprocal control of motility and adherence of uropathogenic Escherichia coli.Infection and immunity · 2012Article
- Stoichiometry of MutS and MutL at unrepaired mismatches in vivo suggests a mechanism of repair.Nucleic acids research · 2012Article
- Article
- Escherichia coli YafP protein modulates DNA damaging property of the nitroaromatic compounds.Nucleic acids research · 2011Article
- Noise-driven heterogeneity in the rate of genetic-variant generation as a basis for evolvability.Genetics · 2010Review
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The presence of repeated DNA sequences is a genomic liability, because interrepeat recombination can result in chromosomal rearrangements. The mismatch repair system prevents recombination between nonidentical repeats, but the mechanism of antirecombination has not been established. Although the MutS protein binds to base pair mismatches in heteroduplex DNA, the role of the MutL protein in preventing recombination is unknown. In a screen designed to identify new cellular functions that suppress deletion formation involving nonidentical DNA repeats, we isolated a mutL mutant having a separation-of-function phenotype. The mutant showed an increased frequency of deletions but not of mutations. The split phenotype is due to a decreased MutL level, indicating that recombination, but not replication editing, is highly sensitive to MutL level. By altering the MutL level, we found that the frequency of deletion-generating recombination is inversely related to the amount of cellular MutL. DNA sequence analysis of the recombined repeats shows that the tolerance of base pair mismatches in heteroduplex DNA is also inversely correlated with MutL level. Unlike recombination, correction of misincorporation errors by mismatch repair is insensitive to fluctuations in MutL level. Overproduction of MutS does not affect either of these phenotypes, suggesting that, unlike MutL, MutS is not limiting for mismatch repair activities. These results indicate that MutL (i) determines effective DNA homology in recombination processes and (ii) fine tunes the process of deletion formation involving repeated, diverged DNA sequences.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.