ArticleProceedings of the National Academy of Sciences of the United States of America2007
beta-Actin regulates platelet nitric oxide synthase 3 activity through interaction with heat shock protein 90.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 45 citations in OpenAlex.
- Platelet redox balance in diabetic patients with hypertension improved by n-3 fatty acids.Diabetes care · 2013Trial
- Thrombocytopenia in patients with inborn errors of immunity.BMC immunology · 2025Article
- Subcellular Localization Guides eNOS Function.International journal of molecular sciences · 2024Review
- β-Actin: An Emerging Biomarker in Ischemic Stroke.Cellular and molecular neurobiology · 2023Review
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- Identification of Partner Proteins of the AlgaeFrontiers in plant science · 2021Article
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- Pharmacologic dissection of the overlapping impact of heat shock protein family members on platelet function.Journal of thrombosis and haemostasis : JTH · 2020Article
- The ACTB Variants and Alcohol Drinking Confer Joint Effect to Ischemic Stroke in Chinese Han Population.Journal of atherosclerosis and thrombosis · 2020Article
- Cytonemes Versus Neutrophil Extracellular Traps in the Fight of Neutrophils with Microbes.International journal of molecular sciences · 2020Review
- Bidirectional cross-regulation between the endothelial nitric oxide synthase and β-catenin signalling pathways.Cardiovascular research · 2014Article
- Regulation of endothelial nitric oxide synthase activity by protein-protein interaction.Current pharmaceutical design · 2014Review
- Genome wide expression analysis suggests perturbation of vascular homeostasis during high altitude pulmonary edema.PloS one · 2014Article
- Simulated microgravity perturbs actin polymerization to promote nitric oxide-associated migration in human immortalized Eahy926 cells.Protoplasma · 2010Article
- Beta-actin association with endothelial nitric-oxide synthase modulates nitric oxide and superoxide generation from the enzyme.The Journal of biological chemistry · 2010Article
- Platelet aggregation responses are critically regulated in vivo by endogenous nitric oxide but not by endothelial nitric oxide synthase.British journal of pharmacology · 2009Article
- Insulin, insulin resistance, and platelet signaling in diabetes.Diabetes care · 2009Article
- Role of Porphyromonas gingivalis SerB in gingival epithelial cell cytoskeletal remodeling and cytokine production.Infection and immunity · 2008Article
Corrections and comments
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Authors and funding
13 authors at 5 institutions in 4 countries.
Funding
Abstract
Cytoskeletal proteins are crucial in maintaining cellular structure and, in certain cell types, also play an essential role in motility and shape change. Nitric oxide (NO) is an important paracrine mediator of vascular and platelet function and is produced in the vasculature by the enzyme NO synthase type 3 (NOS-3). Here, we demonstrate in human platelets that the polymerization state of beta-actin crucially regulates the activation state of NOS-3, and hence NO formation, through altering its binding of heat shock protein 90 (Hsp90). We found that NOS-3 binds to the globular, but not the filamentous, form of beta-actin, and the affinity of NOS-3 for globular beta-actin is, in turn, increased by Hsp90. Formation of this ternary complex among NOS-3, globular beta-actin, and Hsp90, in turn, results in an increase in both NOS activity and cyclic guanosine-3',5'-monophosphate, an index of bioactive NO, as well as an increased rate of Hsp90 degradation, thus limiting the duration for which NOS-3 remains activated. These observations suggest that beta-actin plays a critical role in regulating NO formation and signaling in platelets.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.