ArticleProceedings of the National Academy of Sciences of the United States of America2007
Exposing the core promoter is sufficient to activate transcription and alter coactivator requirement at RNR3.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 30 citations in OpenAlex.
- SWI/SNF and RSC cooperate to reposition and evict promoter nucleosomes at highly expressed genes in yeast.Genes & development · 2018Article
- The transcription factor TpRfx1 is an essential regulator of amylase and cellulase gene expression inBiotechnology for biofuels · 2018Article
- Sas3 and Ada2(Gcn5)-dependent histone H3 acetylation is required for transcription elongation at the de-repressed FLO1 gene.Nucleic acids research · 2017Article
- Nucleosomes Are Essential for Proper Regulation of a Multigated Promoter in Saccharomyces cerevisiae.Genetics · 2016Article
- Genome-wide cooperation by HAT Gcn5, remodeler SWI/SNF, and chaperone Ydj1 in promoter nucleosome eviction and transcriptional activation.Genome research · 2016Article
- Nucleosome positioning in yeasts: methods, maps, and mechanisms.Chromosoma · 2015Review
- Chromatin and transcription in yeast.Genetics · 2012Review
- Multiple sequence-specific factors generate the nucleosome-depleted region on CLN2 promoter.Molecular cell · 2011Article
- Sequential recruitment of SAGA and TFIID in a genomic response to DNA damage in Saccharomyces cerevisiae.Molecular and cellular biology · 2011Article
- Inducible gene expression: diverse regulatory mechanisms.Nature reviews. Genetics · 2010Review
- Functional interplay between chromatin remodeling complexes RSC, SWI/SNF and ISWI in regulation of yeast heat shock genes.Nucleic acids research · 2010Article
- Set2-dependent K36 methylation is regulated by novel intratail interactions within H3.Molecular and cellular biology · 2009Article
- A novel mechanism of antagonism between ATP-dependent chromatin remodeling complexes regulates RNR3 expression.Molecular and cellular biology · 2009Article
- Differential cofactor requirements for histone eviction from two nucleosomes at the yeast PHO84 promoter are determined by intrinsic nucleosome stability.Molecular and cellular biology · 2009Article
- Nucleosome positioning and gene regulation: advances through genomics.Nature reviews. Genetics · 2009Review
- A quantitative model of transcription factor-activated gene expression.Nature structural & molecular biology · 2008Article
- Human SWI/SNF directs sequence-specific chromatin changes on promoter polynucleosomes.Nucleic acids research · 2008Article
- Dissection of coactivator requirement at RNR3 reveals unexpected contributions from TFIID and SAGA.The Journal of biological chemistry · 2008Article
- Yeast Rap1 contributes to genomic integrity by activating DNA damage repair genes.The EMBO journal · 2008Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Chromatin is a formidable barrier to transcription. Nucleosome density is lowest over the regulatory regions of active genes, and many repressed genes have a tightly positioned nucleosome over their core promoter. However, it has not been shown that nucleosome positioning is sufficient for repression or whether disrupting a core promoter nucleosome specifically can activate gene expression in the absence of activating signals. Here we show that disrupting the nucleosome over the core promoter of RNR3 is sufficient to drive preinitiation complex assembly and activate transcription in the absence of activating signals. Remodeling of chromatin over the RNR3 promoter requires the recruitment of the SWI/SNF complex by the general transcription factor TFIID. We found that disrupting the nucleosome over the RNR3 core promoter relieves its dependence on TFIID and SWI/SNF, indicating a functional link between these two complexes. These results suggest that the specific function of TAF(II)s is to direct the chromatin remodeling step through SWI/SNF recruitment, and not core promoter selectivity. Our results indicate that nucleosome placement plays a dominant role in repression and that the ability of the core promoter to position a nucleosome is a major determinant in TAF(II) dependency of genes in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.