ArticleProceedings of the National Academy of Sciences of the United States of America2007
In vitro erythropoiesis from bone marrow-derived progenitors provides a physiological assay for toxic and mutagenic compounds.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
34 citing papers in PubMed.
- Erythropoiesis-inosine metabolic axis failure underlying retinal neurodegeneration in glaucoma: novel diagnoses and therapies.Experimental & molecular medicine · 2026Article
- Reduced GATA1 levels are associated with ineffective erythropoiesis in sickle cell anemia.Haematologica · 2025Article
- Assessment of anemia recovery using peripheral blood smears by deep semi-supervised learning.Annals of hematology · 2025Article
- Article
- IL-18 Binding Protein-Producing Cells Attenuate Anemia in Murine Macrophage Activation Syndrome.Journal of immunology (Baltimore, Md. : 1950) · 2023Article
- Arsenic impairs the lineage commitment of hematopoietic progenitor cells through the attenuation of GATA-2 DNA binding activity.Toxicology and applied pharmacology · 2022Article
- eIF6 rebinding dynamically couples ribosome maturation and translation.Nature communications · 2022Article
- Arsenite and monomethylarsonous acid disrupt erythropoiesis through combined effects on differentiation and survival pathways in early erythroid progenitors.Toxicology letters · 2021Article
- Blockade of IL-22 signaling reverses erythroid dysfunction in stress-induced anemias.Nature immunology · 2021Article
- Article
- Impact of Erythropoietin Production by Erythroblastic Island Macrophages on Homeostatic Murine Erythropoiesis.International journal of molecular sciences · 2020Article
- Inhibition of red blood cell development by arsenic-induced disruption of GATA-1.Scientific reports · 2020Article
- Article
- Omics-Based Platform for Studying Chemical Toxicity Using Stem Cells.Journal of proteome research · 2018Article
- NCOR1 modulates erythroid disorders caused by mutations of thyroid hormone receptor α1.Scientific reports · 2017Article
- Defective erythropoiesis caused by mutations of the thyroid hormone receptor α gene.PLoS genetics · 2017Article
- Modern Approaches to Chemical Toxicity Screening.Current opinion in electrochemistry · 2017Article
- Beneficial Role of Erythrocyte Adenosine A2B Receptor-Mediated AMP-Activated Protein Kinase Activation in High-Altitude Hypoxia.Circulation · 2016Article
- State-of-the-Art Metabolic Toxicity Screening and Pathway Evaluation.Analytical chemistry · 2016Article
- Rps14 haploinsufficiency causes a block in erythroid differentiation mediated by S100A8 and S100A9.Nature medicine · 2016Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
The goal of this study was to create an in vitro cell culture system that captures essential features of the in vivo erythroid micronucleus (MN) genotoxicity assay, thus enabling increased throughput and controlled studies of the hematopoietic DNA damage response. We show that adult bone marrow (BM) cultures respond to erythropoietin, the principal hormone that stimulates erythropoiesis, with physiological erythropoietic proliferation, differentiation, and enucleation. We then show that this in vitro erythropoietic system clearly signals exposure to genotoxicants through erythroid MN formation. Furthermore, we determined that DNA repair-deficient (MGMT(-/-)) BM displayed sensitivity to genotoxic exposure in vivo compared with WT BM and that this phenotypic response was reflected in erythropoietic cultures. These findings suggest that this in vitro erythroid MN assay is capable of screening for genotoxicity on BM in a physiologically reflective manner. Finally, responses to genotoxicants during erythroid differentiation varied with exposure time, demonstrating that this system can be used to study the effect of DNA damage at specific developmental stages.
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