ArticleVascular pharmacology2007
A modified fluorescent microsphere-based approach for determining resting and hyperemic blood flows in individual murine skeletal muscles.
Article in Vascular pharmacology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 21 citations in OpenAlex.
- Endothelial-Specific Reduction in Arf6 Impairs Insulin-Stimulated Vasodilation and Skeletal Muscle Blood Flow Resulting in Systemic Insulin Resistance in Mice.Arteriosclerosis, thrombosis, and vascular biology · 2024Article
- Contrast-Enhanced Ultrasound Reveals Partial Perfusion Recovery After Hindlimb Ischemia as Opposed to Full Recovery by Laser Doppler Perfusion Imaging.Ultrasound in medicine & biology · 2022Article
- Cardiac pericyte reprogramming by MEK inhibition promotes arteriologenesis and angiogenesis of the ischemic heart.The Journal of clinical investigation · 2022Article
- Isometric exercise promotes arteriogenesis in rats after myocardial infarction.Journal of biomedical research · 2021Article
- Epac1Acta physiologica (Oxford, England) · 2019Article
- Validation of diffuse correlation spectroscopy sensitivity to nicotinamide-induced blood flow elevation in the murine hindlimb using the fluorescent microsphere technique.Journal of biomedical optics · 2018Article
- Epoxyeicosatrienoic acids mediate insulin-mediated augmentation in skeletal muscle perfusion and blood volume.American journal of physiology. Endocrinology and metabolism · 2014Article
- Image-based assessment of microvascular function and structure in collagen XV- and XVIII-deficient mice.The Journal of physiology · 2014Article
- Microvascular repair: post-angiogenesis vascular dynamics.Microcirculation (New York, N.Y. : 1994) · 2012Review
- Structural remodeling of the mouse gracilis artery: coordinated changes in diameter and medial area maintain circumferential stress.Microcirculation (New York, N.Y. : 1994) · 2012Article
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2 authors at 1 institution in 1 country.
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Abstract
The goal of this study was to develop a modified fluorescent microsphere-based approach for measuring resting and hyperemic blood flows in individual mouse skeletal muscles. Absolute resting blood flow in the left gracilis posterior was 1.04+/-0.12 ml x min(-1).g(-1), while functional hyperemia following muscle activity was 5.94+/-1.33 ml x min(-1) x g(-1). Measuring absolute blood flow requires sampling arterial blood that serves as a flow-rate and concentration reference to the fluorescent microsphere (FMS) content in the tissue-of-interest for calculating the flow value. Because sampling arterial blood can impair cardiovascular function in the mouse, we also modified our FMS approach to determine relative blood flows in the left gracilis posterior by using the contralateral muscle as our reference in blood flow calculations. Absolute and relative hyperemia measurements detect similar increases in blood flow - 521.93+/-216.76% and 555.24+/-213.82%, respectively. However, sampling arterial blood during absolute blood flow measurements significantly decreased mean arterial pressure from the beginning to the end of our experiments, from 102.7+/-2.18 to 75.5+/-9.71 mm Hg. This decrease was not seen when measuring relative blood flows. This approach provides critical advantages over contemporary blood flow measurement approaches by allowing blood flow measurements in small and non-superficial tissues.
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