ArticleThe Plant cell2007
Increasing plasma membrane phosphatidylinositol(4,5)bisphosphate biosynthesis increases phosphoinositide metabolism in Nicotiana tabacum.
Article in The Plant cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 70 citations in OpenAlex.
- Genome-wide association mapping of seed oligosaccharides in chickpea.Frontiers in plant science · 2022Article
- Plasma membrane phospholipid signature recruits the plant exocyst complex via the EXO70A1 subunit.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Galactolipid and Phospholipid Profile and Proteome Alterations in Soybean Leaves at the Onset of Salt Stress.Frontiers in plant science · 2021Article
- A Plant-Specific N-terminal Extension Reveals Evolutionary Functional Divergence within Translocator Proteins.iScience · 2020Article
- Perturbing phosphoinositide homeostasis oppositely affects vascular differentiation inDevelopment (Cambridge, England) · 2017Article
- Article
- Seed Biofortification and Phytic Acid Reduction: A Conflict of Interest for the Plant?Plants (Basel, Switzerland) · 2015Review
- Do phosphoinositides regulate membrane water permeability of tobacco protoplasts by enhancing the aquaporin pathway?Planta · 2015Article
- Effect of polyelectrolyte adsorption on lateral distribution and dynamics of anionic lipids: a Monte Carlo study of a coarse-grain model.European biophysics journal : EBJ · 2014Article
- Compositional redistribution and dynamic heterogeneity in mixed lipid membrane induced by polyelectrolyte adsorption: effects of chain rigidity.The European physical journal. E, Soft matter · 2014Article
- Increasing Phosphatidylinositol (4,5)-Bisphosphate Biosynthesis Affects Basal Signaling and Chloroplast Metabolism in Arabidopsis thaliana.Plants (Basel, Switzerland) · 2014Article
- Salicylic acid modulates levels of phosphoinositide dependent-phospholipase C substrates and products to remodel the Arabidopsis suspension cell transcriptome.Frontiers in plant science · 2014Article
- PIP kinases and their role in plant tip growing cells.Plant signaling & behavior · 2012Review
- Increasing phosphatidylinositol (4,5) bisphosphate biosynthesis affects plant nuclear lipids and nuclear functions.Plant physiology and biochemistry : PPB · 2012Article
- A suggested model for potato MIVOISAP involving functions of central carbohydrate and amino acid metabolism, as well as actin cytoskeleton and endocytosis.Plant signaling & behavior · 2010Article
- Phosphoinositides regulate clathrin-dependent endocytosis at the tip of pollen tubes in Arabidopsis and tobacco.The Plant cell · 2010Article
- At the poles across kingdoms: phosphoinositides and polar tip growth.Protoplasma · 2010Review
- Proteomic analysis of blue light-induced twining response in Cuscuta australis.Plant molecular biology · 2010Article
- Phospholipid signaling responses in salt-stressed rice leaves.Plant & cell physiology · 2009Article
- Switches in nutrient and inositol signaling.Plant signaling & behavior · 2009Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
A genetic approach was used to increase phosphatidylinositol(4,5)bisphosphate [PtdIns(4,5)P2] biosynthesis and test the hypothesis that PtdInsP kinase (PIPK) is flux limiting in the plant phosphoinositide (PI) pathway. Expressing human PIPKIalpha in tobacco (Nicotiana tabacum) cells increased plasma membrane PtdIns(4,5)P2 100-fold. In vivo studies revealed that the rate of 32Pi incorporation into whole-cell PtdIns(4,5)P2 increased >12-fold, and the ratio of [3H]PtdInsP2 to [3H]PtdInsP increased 6-fold, but PtdInsP levels did not decrease, indicating that PtdInsP biosynthesis was not limiting. Both [3H]inositol trisphosphate and [3H]inositol hexakisphosphate increased 3-and 1.5-fold, respectively, in the transgenic lines after 18 h of labeling. The inositol(1,4,5)trisphosphate [Ins(1,4,5)P3] binding assay showed that total cellular Ins(1,4,5)P3/g fresh weight was >40-fold higher in transgenic tobacco lines; however, even with this high steady state level of Ins(1,4,5)P3, the pathway was not saturated. Stimulating transgenic cells with hyperosmotic stress led to another 2-fold increase, suggesting that the transgenic cells were in a constant state of PI stimulation. Furthermore, expressing Hs PIPKIalpha increased sugar use and oxygen uptake. Our results demonstrate that PIPK is flux limiting and that this high rate of PI metabolism increased the energy demands in these cells.
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