ArticleBiophysical journal2007
Formation of ceramide/sphingomyelin gel domains in the presence of an unsaturated phospholipid: a quantitative multiprobe approach.
Article in Biophysical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 122 citations in OpenAlex.
- Ceramide and the membrane-fusion activity of LC3/GABARAP autophagy proteins.Cellular and molecular life sciences : CMLS · 2025Article
- Lipids in Mitochondrial Macroautophagy: Phase Behavior of Bilayers Containing Cardiolipin and Ceramide.International journal of molecular sciences · 2023Article
- Fluorescent ProbesMolecules (Basel, Switzerland) · 2023Article
- Effect of Cisplatin and Its Cationic Analogues in the Phase Behavior and Permeability of Model Lipid Bilayers.Molecular pharmaceutics · 2023Article
- Sphingomyelinase-Mediated Multitimescale Clustering of Ganglioside GM1 in Heterogeneous Lipid Membranes.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Sphingomyelin Acyl Chains Influence the Formation of Sphingomyelin- and Cholesterol-Enriched Domains.Biophysical journal · 2020Article
- C24:0 and C24:1 sphingolipids in cholesterol-containing, five- and six-component lipid membranes.Scientific reports · 2020Article
- Impact of Acyl Chain Mismatch on the Formation and Properties of Sphingomyelin-Cholesterol Domains.Biophysical journal · 2019Article
- Lateral Segregation of Palmitoyl Ceramide-1-Phosphate in Simple and Complex Bilayers.Biophysical journal · 2019Article
- Nonlamellar-Phase-Promoting Colipids Enhance Segregation of Palmitoyl Ceramide in Fluid Bilayers.Biophysical journal · 2019Article
- Natural Ceramides and Lysophospholipids Cosegregate in Fluid Phosphatidylcholine Bilayers.Biophysical journal · 2019Article
- Sphingolipids and lipid rafts: Novel concepts and methods of analysis.Chemistry and physics of lipids · 2018Review
- Sphingomyelin Stereoisomers Reveal That Homophilic Interactions Cause Nanodomain Formation.Biophysical journal · 2018Article
- The many faces (and phases) of ceramide and sphingomyelin II - binary mixtures.Biophysical reviews · 2017Review
- Development of lysosome-mimicking vesicles to study the effect of abnormal accumulation of sphingosine on membrane properties.Scientific reports · 2017Article
- Bilayer Interactions among Unsaturated Phospholipids, Sterols, and Ceramide.Biophysical journal · 2017Article
- The Long-Chain Sphingoid Base of Ceramides Determines Their Propensity for Lateral Segregation.Biophysical journal · 2017Article
- Three-Phase Coexistence in Lipid Membranes.Biophysical journal · 2017Article
- Glucosylceramide Reorganizes Cholesterol-Containing Domains in a Fluid Phospholipid Membrane.Biophysical journal · 2016Article
- Influence of Hydroxylation, Chain Length, and Chain Unsaturation on Bilayer Properties of Ceramides.Biophysical journal · 2015Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To better understand how ceramide modulates the biophysical properties of the membrane, the interactions between palmitoyl-ceramide (PCer) and palmitoyl-sphingomyelin (PSM) were studied in the presence of the fluid phospholipid palmitoyl-oleoyl-phosphatidylcholine (POPC) in membrane model systems. The use of two fluorescent membrane probes distinctly sensitive to lipid phases allowed a thorough biophysical characterization of the ternary system. In these mixtures, PCer recruits POPC and PSM in the fluid phase to form extremely ordered and compact gel domains. Gel domain formation by low PCer mol fraction (up to 12 mol %) is enhanced by physiological PSM levels (approximately 20-30 mol % total lipid). For higher PSM content, a three-phase situation, consisting of fluid (POPC-rich)/gel (PSM-rich)/gel (PCer-rich) coexistence, is clearly shown. To determine the fraction of each phase a quantitative method was developed. This allowed establishing the complete ternary phase diagram, which helps to predict PCer-rich gel domain formation and explains its enhancement through PSM/PCer interactions.
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