ArticleStem cells (Dayton, Ohio)2007
Human amniotic epithelial cells as novel feeder layers for promoting ex vivo expansion of limbal epithelial progenitor cells.
Article in Stem cells (Dayton, Ohio), 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- Constitutive expression of pentraxin 3 (PTX3) protein by human amniotic membrane cells leads to formation of the heavy chain (HC)-hyaluronan (HA)-PTX3 complex.The Journal of biological chemistry · 2014Trial
- The Role of Genetically Modified Human Feeder Cells in Maintaining the Integrity of Primary Cultured Human Deciduous Dental Pulp Cells.Journal of clinical medicine · 2022Article
- A Survey and Critical Evaluation of Isolation, Culture, and Cryopreservation Methods of Human Amniotic Epithelial Cells.Cell cycle (Georgetown, Tex.) · 2022Review
- Niche regulation of limbal epithelial stem cells: HC-HA/PTX3 as surrogate matrix niche.Experimental eye research · 2020Review
- Article
- Proliferation and survival of human amniotic epithelial cells during their hepatic differentiation.PloS one · 2018Article
- Method and key points for isolation of human amniotic epithelial cells with high yield, viability and purity.BMC research notes · 2017Article
- Diagnosis of limbal stem cell deficiency based on corneal epithelial thickness measured on anterior segment optical coherence tomography.Indian journal of ophthalmology · 2017Article
- Human amnion-derived mesenchymal stem cells protect against UVA irradiation-induced human dermal fibroblast senescence, in vitro.Molecular medicine reports · 2017Article
- Article
- Comparative analysis of different feeder layers with 3T3 fibroblasts for culturing rabbits limbal stem cells.International journal of ophthalmology · 2017Article
- Effects of amniotic epithelial cell transplantation in endothelial injury.Interventional medicine & applied science · 2016Review
- An Ultra-thin Amniotic Membrane as Carrier in Corneal Epithelium Tissue-Engineering.Scientific reports · 2016Article
- Corneal recovery in a rabbit limbal stem cell deficiency model by autologous grafts of tertiary outgrowths from cultivated limbal biopsy explants.Molecular vision · 2016Article
- Feeder Layer Cell Actions and Applications.Tissue engineering. Part B, Reviews · 2015Review
- Optimal isolation and xeno-free culture conditions for limbal stem cell function.Investigative ophthalmology & visual science · 2014Article
- Optimization of Ex Vivo Expansion of Limbal Epithelial Progenitors by Maintaining Native Niche Cells on Denuded Amniotic Membrane.Translational vision science & technology · 2013Article
- Presence of native limbal stromal cells increases the expansion efficiency of limbal stem/progenitor cells in culture.Experimental eye research · 2013Article
- Kinetics of expansion of human limbal epithelial progenitor cells in primary culture of explants without feeders.PloS one · 2013Article
- Pax6 downregulation mediates abnormal lineage commitment of the ocular surface epithelium in aqueous-deficient dry eye disease.PloS one · 2013Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Human amniotic epithelial cells (HAECs) are a unique embryonic cell source that potentially can be used as feeder layers for expanding different types of stem cells. In vivo, HAECs uniformly expressed pan-cytokeratins (pan-CK) and heterogeneously expressed vimentin (Vim). The two phenotypes expressing either pan-CK(+)/Vim(+) or pan-CK(+)/Vim(-) were maintained in serum-free media with high calcium. In contrast, all HAECs became pan-CK(+)/Vim(+) in serum-containing media, which also promoted HAEC proliferation for at least eight passages, especially supplemented with epidermal growth factor and insulin. Mitomycin C-arrested HAEC feeder layers were more effective in promoting clonal growth of human limbal epithelial progenitors than conventional 3T3 murine feeder layers. Cells in HAEC-supported clones were uniformly smaller, sustained more proliferation, and expressed less CK12 and connexin 43 but higher levels of stem cell-associated markers such as p63, Musashi-1, and ATP-binding cassette subfamily G2 than those of 3T3-supported clones. Subculturing of clonally expanded limbal progenitors from HAEC feeder layers, but not from 3T3 feeder layers, gave rise to uniformly p63-positive epithelial progenitor cells as well as nestin-positive neuronal-like progenitors. Collectively, these results indicated that HAECs can be used as a human feeder layer equivalent for more effective ex vivo expansion of adult epithelial stem cells from the human limbus. Disclosure of potential conflicts of interest is found at the end of this article.
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