ArticleMolecular microbiology2007
Identification of mutations that alter the gating of the Escherichia coli mechanosensitive channel protein, MscK.
Article in Molecular microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- The bacterial mechanosensitive channel MscM gates through concerted changes in its transmembrane and cytoplasmic domains.Nature communications · 2026Article
- MscM uses a novel gating mechanism for bacterial mechanosensitive channels.bioRxiv : the preprint server for biology · 2026Article
- Identification of theMicrobiology (Reading, England) · 2025Article
- Structural basis for mechanotransduction in a potassium-dependent mechanosensitive ion channel.Nature communications · 2022Article
- The Structure of YnaI Implies Structural and Mechanistic Conservation in the MscS Family of Mechanosensitive Channels.Structure (London, England : 1993) · 2015Article
- The evolutionary 'tinkering' of MscS-like channels: generation of structural and functional diversity.Pflugers Archiv : European journal of physiology · 2015Review
- Arabidopsis MSL10 has a regulated cell death signaling activity that is separable from its mechanosensitive ion channel activity.The Plant cell · 2014Article
- Selectivity mechanisms in MscS-like channels: From structure to function.Channels (Austin, Tex.) · 2014Article
- MscS-like mechanosensitive channels in plants and microbes.Biochemistry · 2013Review
- Identification and analysis of putative homologues of mechanosensitive channels in pathogenic protozoa.PloS one · 2013Article
- Functional analysis of conserved motifs in the mechanosensitive channel homolog MscS-Like2 from Arabidopsis thaliana.PloS one · 2012Article
- Mechanosensitive channels: what can they do and how do they do it?Structure (London, England : 1993) · 2011Review
- Defining the role of the tension sensor in the mechanosensitive channel of small conductance.Biophysical journal · 2011Article
- Genetic screen for potassium leaky small mechanosensitive channels (MscS) in Escherichia coli: recognition of cytoplasmic β domain as a new gating element.The Journal of biological chemistry · 2011Article
- The LysR-type transcriptional regulator LeuO controls expression of several genes in Salmonella enterica serovar Typhi.Journal of bacteriology · 2008Article
- Recent characterizations of MscS and its homologs provide insight into the basis of ion selectivity in mechanosensitive channels.Channels (Austin, Tex.)Article
Corrections and comments
- Erratum issuedJeong, Hochterl [corrected to Jeong, Hotcherl]
Authors and funding
6 authors.
Funding
Abstract
Mechanosensitive channels allow bacteria to survive rapid increases in turgor pressure. Substantial questions remain as to how these channels sense and respond to mechanical stress. Here we describe a set of mutants with alterations in their MscK channel protein. The mutants were detected fortuitously by their enhanced ability to modify the accumulation of quinolinic acid. Some amino acid changes lie in the putative pore region of MscK, but others affect sequences that lie amino-terminal to the domain aligning with MscS. We demonstrate that the alterations in MscK cause the channel to open more frequently in the absence of excessive mechanical stress. This is manifested in changes in sensitivity to external K(+) by cells expressing the mutant proteins. Single-channel analysis highlighted a range of gating behaviours: activation at lower pressures than the wild type, inability to achieve the fully open state or a modified requirement for K(+). Thus, the dominant uptake phenotype of these mutants may result from a defect in their ability to regulate the gating of MscK. The locations of the substituted residues suggest that the overall gating mechanism of MscK is comparable to that of MscS, but with subtleties introduced by the additional protein sequences in MscK.
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