ArticleClinical rheumatology2008
Prevalence of ZAP-70, LAT, SLP-76, and DNA methyltransferase 1 expression in CD4+ T cells of patients with systemic lupus erythematosus.
Article in Clinical rheumatology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 42 citations in OpenAlex.
- Methyl-donor supplementation in women with systemic lupus erythematosus with different nutritional status: the protocol for a randomised, double-blind, placebo-controlled trial.Lupus science & medicine · 2024Article
- Egr2 Deletion in Autoimmune-Prone C57BL6/lpr Mice Suppresses the Expression of Methylation-Sensitive Dlk1-Dio3 Cluster MicroRNAs.ImmunoHorizons · 2023Article
- The Molecular Pathophysiology of Psoriatic Arthritis-The Complex Interplay Between Genetic Predisposition, Epigenetics Factors, and the Microbiome.Frontiers in molecular biosciences · 2021Review
- Epigenetics in SLE.Current rheumatology reports · 2017Review
- DNA methylation in systemic lupus erythematosus.Epigenomics · 2017Review
- Epigenetics of multiple sclerosis: an updated review.Neuromolecular medicine · 2015Review
- Metabolic control of the epigenome in systemic Lupus erythematosus.Autoimmunity · 2014Review
- Vitamin D receptor gene BsmI, FokI, ApaI and TaqI polymorphisms and the risk of systemic lupus erythematosus.Molecular biology reports · 2013Article
- Prevalence of the NKG2D Thr72Ala polymorphism in patients with systemic lupus erythematosus.Molecular biology reports · 2012Article
- The role of epigenetic mechanisms and processes in autoimmune disorders.Biologics : targets & therapy · 2012Article
- cAMP-responsive element modulator (CREM)α protein signaling mediates epigenetic remodeling of the human interleukin-2 gene: implications in systemic lupus erythematosus.The Journal of biological chemistry · 2011Article
- Epigenetic mechanisms in systemic lupus erythematosus and other autoimmune diseases.Trends in molecular medicine · 2011Review
- Activation of the signaling cascade in response to T lymphocyte receptor stimulation and prostanoids in a case of cutaneous lupus.North American journal of medical sciences · 2011Article
- SDF1-3' G801A polymorphisms in Polish patients with systemic lupus erythematosus.Molecular biology reports · 2010Article
- Monocyte chemoattractant protein-1 -2518 A/G single nucleotide polymorphism might be associated with renal disease and thrombocytopenia of SLE.Journal of biomedicine & biotechnology · 2010Article
- IL-18 105 A>C polymorphism contributes to renal manifestations in patients with SLE.Rheumatology international · 2009Article
- Genetic variation in the interleukin-10 gene promoter in Polish patients with systemic lupus erythematosus.Rheumatology international · 2009Article
- Catalase -262C>T polymorphism in systemic lupus erythematosus in Poland.Rheumatology international · 2008Article
- Manganese superoxide dismutase Ala-9Val mitochondrial targeting sequence polymorphism in systemic lupus erythematosus in Poland.Clinical rheumatology · 2008Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
T cells from systemic lupus erythematosus (SLE) patients exhibit defective function of CD4(+) T cells that can be responsible for improper activation of B cells and antibody biosynthesis against host antigens. We compared the level of ZAP-70, LAT, and SLP-76, transcripts and proteins in CD4(+) T cells from SLE patients (n = 22) and healthy individuals (n = 15). We also determined DNA methyltransferase 1 (DNMT1) protein content in CD4(+) T cells of SLE patients. The CD4(+) T cells were isolated by positive biomagnetic separation technique. The quantitative analysis of messenger RNA (mRNA) was performed by reverse transcription and real-time quantitative polymerase chain reaction (RQ-PCR) SYBR Green I system. The protein level in the CD4(+) T cells was determined by Western blotting analysis. We found that the LAT protein level was significantly higher in SLE CD4(+) T cells than in controls (P = 0.006). Western blot analysis revealed that ZAP-70 protein content in SLE CD4(+) T cells may be reciprocally correlated with disease activity expressed in SLEDAI scale (R = -0.623, P = 0.002) or number of affected organ systems (R = -0.549, P = 0.008). We also observed reciprocal correlation between DNMT1 protein content in CD4(+) T cells and disease activity scored with SLEDAI scale (R = -0.779, P = 0.001) or number of affected organ systems (R = -0.617, P = 0.019), respectively. Our findings might indicate that LAT, ZAP-70, and DNMT1 protein levels in CD4(+) T cells can be associated with SLE disease.
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