ArticleJournal of fluorescence2007
Visualization of arginine influx into plant cells using a specific FRET-sensor.
Article in Journal of fluorescence, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 59 citations in OpenAlex.
- Optical bio/chemical sensors for vitamin BBeilstein journal of nanotechnology · 2025Review
- Synthetic Biology in Plants, a Boon for Coming Decades.Molecular biotechnology · 2021Review
- FRET-Based Genetically Encoded Sensor to Monitor Silver Ions.ACS omega · 2021Article
- Designing and Development of FRET-Based Nanosensor for Real Time Analysis of N-Acetyl-5-Neuraminic Acid in Living Cells.Frontiers in nutrition · 2021Article
- Article
- The Role of Amino Acids in Neurotransmission and Fluorescent Tools for Their Detection.International journal of molecular sciences · 2020Review
- In Vitro Analytical Approaches to Study Plant Ligand-Receptor Interactions.Plant physiology · 2020Review
- Differential Contribution of P5CS Isoforms to Stress Tolerance in Arabidopsis.Frontiers in plant science · 2020Article
- Engineering genetically encoded FRET-based nanosensors for real time display of arsenic (AsScientific reports · 2019Article
- Converting a Periplasmic Binding Protein into a Synthetic Biosensing Switch through Domain Insertion.BioMed research international · 2019Review
- Genetically Encoded Fluorescent Biosensors Illuminate the Spatiotemporal Regulation of Signaling Networks.Chemical reviews · 2018Review
- Fluorescent Proteins for Investigating Biological Events in Acidic Environments.International journal of molecular sciences · 2018Review
- Designing, construction and characterization of genetically encoded FRET-based nanosensor for real time monitoring of lysine flux in living cells.Journal of nanobiotechnology · 2016Article
- Real-time functional characterization of cationic amino acid transporters using a new FRET sensor.Pflugers Archiv : European journal of physiology · 2016Article
- Construction of a robust and sensitive arginine biosensor through ancestral protein reconstruction.Protein science : a publication of the Protein Society · 2015Article
- The putative Cationic Amino Acid Transporter 9 is targeted to vesicles and may be involved in plant amino acid homeostasis.Frontiers in plant science · 2015Article
- Development of FRET biosensors for mammalian and plant systems.Protoplasma · 2014Review
- A genetically encoded, high-signal-to-noise maltose sensor.Proteins · 2011Article
- H-independent glutamine transport in plant root tips.PloS one · 2010Article
- Comparison of quantitative metabolite imaging tools and carbon-13 techniques for fluxomics.Methods in molecular biology (Clifton, N.J.) · 2009Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Amino acids are not only the building blocks of proteins, but are the metabolic precursors of a variety of primary and secondary metabolites. In order to detect and visualize how plants transport, sense, and store amino acids with sub-cellular specificity, chimeric fluorescent proteins that respond to changes in amino acid concentrations were constructed. The reporter element of these sensors consists of a periplasmic bacterial protein that undergoes large, non-enzymatic conformational changes upon binding of its substrate. The receptor protein was attached to ECFP and an environmentally insensitive YFP derivative at opposite ends. Fluorescence resonance energy transfer changes were specifically observed after addition of arginine and to a lesser extent ornithine. The recombinant sensor showed a concentration-dependent increase in the fluorescence ratio with an apparent in vitro affinity for arginine of approximately 2 mM. A mutation in the binding pocket lowered the affinity and decreased the specificity. When expressed in E. coli, an increase in the fluorescence ratio was specifically detected after exposure to arginine and ornithine. Transient expression of the sensor in plant cell protoplasts and stable expression in Arabidopsis roots revealed specific fluorescence changes upon addition of arginine. The analysis suggests that fluorescent amino acid sensors may be versatile tools for studying the in vivo dynamics of metabolism and compartmentalization in plants.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.