ArticlePlant cell reports2007
High expression of transgene protein in Spirodela.
Article in Plant cell reports, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 67 citations in OpenAlex.
- Duckweeds: from fundamental biology to a sustainable plant chassis for biotechnology.Advanced biotechnology · 2026Review
- Duckweeds: Model organisms for research on plant sterols and steroids.Plant biology (Stuttgart, Germany) · 2026Review
- Systematic Optimization Enables Near-Perfect In Vitro Transformation Efficiencies forbioRxiv : the preprint server for biology · 2025Article
- The "Duckweed Dip": AquaticACS synthetic biology · 2024Article
- Transcriptomic and physiological analysis of the response of Spirodela polyrrhiza to sodium nitroprusside.BMC plant biology · 2024Article
- Engineering triacylglycerol accumulation in duckweed (Lemna japonica).Plant biotechnology journal · 2023Article
- Plant-made pharmaceuticals: exploring studies for the production of recombinant protein in plants and assessing challenges ahead.Plant biotechnology reports · 2023Review
- Rapid and Highly Efficient Genetic Transformation and Application of Interleukin-17B Expressed in Duckweed as Mucosal Vaccine Adjuvant.Biomolecules · 2022Article
- Article
- RobustFrontiers in bioengineering and biotechnology · 2021Article
- Article
- Efficient genetic transformation and CRISPR/Cas9-mediated genome editing in Lemna aequinoctialis.Plant biotechnology journal · 2019Article
- Frond transformation system mediated by Agrobacterium tumefaciens for Lemna minor.Plant molecular biology · 2018Article
- Development of a New Marker System for Identification ofInternational journal of genomics · 2017Article
- State of research in the field of the creation of plant vaccines for veterinary use.Russian journal of plant physiology: a comprehensive Russian journal on modern phytophysiology · 2015Review
- Duckweed (Lemna minor) as a model plant system for the study of human microbial pathogenesis.PloS one · 2010Article
- Genetic structure of the genus Lemna L. (Lemnaceae) as revealed by amplified fragment length polymorphism.Planta · 2010Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The monocot family Lemnaceae (duckweed) is composed of small, edible, aquatic plants. Spirodela oligorrhiza SP is a duckweed with a biomass doubling time of about 2 days under controlled, axenic conditions. Stably transformed Spirodela plants were obtained following co-cultivation of regenerative calli with Agrobacterium tumefaciens. GFP activity was successfully monitored in different subcellular compartments of the plant and correlated with different targeting sequences. Transgenic lines were followed for a period of at least 18 months and more than 180 vegetative doublings (generations). The lines are stable in morphology, growth rate, transgene expression, and activity as measured by DNA-DNA and immunoblot hybridizations, fluorescence activity measurements, and antibiotic resistance. The level of transgene expression is a function of leader sequences rather than transgene copy number. A stable, transgenic, GFP expression level >25% of total soluble protein is demonstrated for the S. oligorrhiza system, making it among the higher expressing systems for nuclear transformation in a higher plant.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.