ArticlePlant molecular biology2007
Transgenic cassava resistance to African cassava mosaic virus is enhanced by viral DNA-A bidirectional promoter-derived siRNAs.
Article in Plant molecular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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28 citing papers in PubMed, 103 citations in OpenAlex.
- Advancements and strategies of genetic improvement in cassava (Horticulture research · 2025Article
- Geminivirus diseases of legumes in India: current status and approaches for management.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Review
- Article
- CRISPR/Cas9: an advanced platform for root and tuber crops improvement.Frontiers in genome editing · 2023Review
- Review
- RNA silencing ofBiotechnology reports (Amsterdam, Netherlands) · 2019Article
- Expression of short hairpin RNA (shRNA) targeting AC2 gene of3 Biotech · 2019Article
- Review
- Genetic Transformation of Recalcitrant Cassava by Embryo Selection and Increased Hormone Levels.Methods and protocols · 2018Article
- A molecular insight into papaya leaf curl-a severe viral disease.Protoplasma · 2017Review
- Review
- Formation of friable embryogenic callus in cassava is enhanced under conditions of reduced nitrate, potassium and phosphate.PloS one · 2017Article
- Small RNA Based Genetic Engineering for Plant Viral Resistance: Application in Crop Protection.Frontiers in microbiology · 2017Review
- RNAi mediated broad-spectrum transgenic resistance in Nicotiana benthamiana to chilli-infecting begomoviruses.Plant cell reports · 2015Article
- Application of molecular antiviral compounds: novel approach for durable resistance against geminiviruses.Molecular biology reports · 2015Review
- Resistance to Sri Lankan cassava mosaic virus (SLCMV) in genetically engineered cassava cv. KU50 through RNA silencing.PloS one · 2015Article
- How can plant DNA viruses evade siRNA-directed DNA methylation and silencing?International journal of molecular sciences · 2013Review
- Article
- Robust transformation procedure for the production of transgenic farmer-preferred cassava landraces.Plant methods · 2012Article
- A sweetpotato SRD1 promoter confers strong root-, taproot-, and tuber-specific expression in Arabidopsis, carrot, and potato.Transgenic research · 2012Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Expression of double-stranded RNA (dsRNA) homologous to virus sequences can effectively interfere with RNA virus infection in plant cells by triggering RNA silencing. Here we applied this approach against a DNA virus, African cassava mosaic virus (ACMV), in its natural host cassava. Transgenic cassava plants were developed to express small interfering RNAs (siRNA) from a CaMV 35S promoter-controlled, intron-containing dsRNA cognate to the common region-containing bidirectional promoter of ACMV DNA-A. In two of three independent transgenic lines, accelerated plant recovery from ACMV-NOg infection was observed, which correlates with the presence of transgene-derived siRNAs 21-24 nt in length. Overall, cassava mosaic disease symptoms were dramatically attenuated in these two lines and less viral DNA accumulation was detected in their leaves than in those of wild-type plants. In a transient replication assay using leaf disks from the two transgenic lines, strongly reduced accumulation of viral single-stranded DNA was observed. Our study suggests that a natural RNA silencing mechanism targeting DNA viruses through production of virus-derived siRNAs is turned on earlier and more efficiently in transgenic plants expressing dsRNA cognate to the viral promoter and common region.
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