Trial reportThe American journal of clinical nutrition2007
Inadequate protein intake affects skeletal muscle transcript profiles in older humans.
Trial report in The American journal of clinical nutrition, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it, 79 citations in OpenAlex.
- Factors influencing the efficacy of nutritional interventions on muscle mass in older adults: a systematic review and meta-analysis.Nutrition reviews · 2021Pooled it
- A Novel Dietary Intervention Reduces Circulatory Branched-Chain Amino Acids by 50%: A Pilot Study of Relevance for Obesity and Diabetes.Nutrients · 2020Trial
- Community-based intervention to improve dietary habits and promote physical activity among older adults: a cluster randomized trial.BMC geriatrics · 2013Trial
- The skeletal muscle transcript profile reflects accommodative responses to inadequate protein intake in younger and older males.The Journal of nutritional biochemistry · 2010Trial
- Sex-specific physical activity thresholds influencing muscle function in older adults with XX genotype of ACTN3 R577X.Scientific reports · 2026Article
- Malnutrition and Sarcopenia in Gastrointestinal Motility Disorders: Mechanisms, Assessment, and Management.Current gastroenterology reports · 2026Review
- The Influence of Acute Beta-Hydroxy Beta-Methylbutyrate (HMB) Ingestion on the Human Skeletal Muscle Transcriptome.Nutrients · 2026Article
- Effect of Goat Meat on Muscle Atrophy Induced by Dexamethasone in Mice.Food science of animal resources · 2025Article
- The Impact of Bariatric Surgery on Nitrogen Balance at Six months Post-surgery.Obesity surgery · 2024Article
- Transcriptomic Analysis of Human Skeletal Muscle in Response to Aerobic Exercise and Protein Intake.Nutrients · 2023Article
- Increased Intake of Omega-3 Polyunsaturated Fatty Acids Is Associated with Reduced Odds of Low Hand Grip Strength in Korean Adults.Nutrients · 2023Article
- Association between food texture levels consumed and the prevalence of malnutrition and sarcopenia in older patients after stroke.European journal of clinical nutrition · 2022Article
- Association between total protein intake and low muscle mass in Korean adults.BMC geriatrics · 2022Article
- Guava Fruit andNutrients · 2022Article
- Association between frailty and dietary quality in community-dwelling elderly: data from the 6th Korea National Health and Nutrition Examination Survey (2014-2015).Journal of clinical biochemistry and nutrition · 2021Article
- Review
- Protein Availability and Satellite Cell Dynamics in Skeletal Muscle.Sports medicine (Auckland, N.Z.) · 2018Review
- Does Protein Intake Affect Metabolic Risk Factors among Older Adults in Korea?Journal of obesity & metabolic syndrome · 2017Article
- Frailty and nutrition: searching for evidence.The journal of nutrition, health & aging · 2015Review
- Distributional fold change test - a statistical approach for detecting differential expression in microarray experiments.Algorithms for molecular biology : AMB · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
backgroundInadequate dietary protein intake causes adverse changes in the morphology and function of skeletal muscle. These changes may be reflected in early alterations in muscle messenger RNA levels.
objectiveThis study assessed whether inadequate protein intake differentially affects skeletal muscle transcript concentrations and expression profiles in older adults.
designTwenty-one older men and women (aged 55-80 y) consumed controlled diets that provided 1.2 g protein x kg(-1) x d(-1) (adequate protein) for 1 wk and then were randomly assigned to consume either 0.5 g protein x kg(-1) x d(-1) [inadequate protein (IP) group; n=11] or 1.2 g protein x kg(-1) x d(-1) (control group; n=10) for a second week. RNA was isolated from fasting-state vastus lateralis biopsy samples obtained at the end of each period, and transcript levels in the IP group were measured by using microarray analysis. Changes in selected transcript levels were confirmed by real-time polymerase chain reaction in both groups.
resultsAnalysis of variance showed 529 differentially expressed transcripts (P<0.05) after inadequate protein intake. Using the false discovery rate (FDR) correction to adjust for multiple comparisons, we observed that 85 transcripts were differentially expressed: 54 were up-regulated and 31 were down-regulated. The differentially expressed transcripts were in functional classes for immune, inflammatory, and stress responses (predominantly up-regulated); contraction, movement, and development (up-regulated); extracellular connective tissue (up-regulated); energy metabolism (down-regulated); protein synthesis (down-regulated); and proliferation (down-regulated). Diet-related differences in the expression of 9 transcripts were cross-validated by using real-time polymerase chain reaction.
conclusionThe results document changes in skeletal muscle transcript levels induced by short-term inadequate protein intakes in older humans that might precede adverse metabolic, functional, and structural events, including muscle wasting.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.