ArticleBMC genomics2007
Sequence-indexed mutations in maize using the UniformMu transposon-tagging population.
Article in BMC genomics, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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Who cites it
64 citing papers in PubMed.
- Constitutive and tissue-specific target site preferences of the maize Mutator transposon.bioRxiv : the preprint server for biology · 2026Article
- The maize mitogen-activated protein kinase kinase kinase gene ZmMAPKKK45 is associated with multiple disease resistance.The New phytologist · 2026Article
- Quick creation and mapping of EMS-induced maize kernel mutants identifies classical gene ZmBT1 and novel gene ZmTOP6A.The plant genome · 2026Article
- Characterization of two Mutator insertion alleles ofmicroPublication biology · 2026Article
- Large DNA and protein language models enhance discovery of deleterious mutations in maize.Genome biology · 2025Article
- Interplay between autophagy, the unfolded protein response and the cytoplasmic heat stress response during heat stress in maize.BMC plant biology · 2025Article
- Natural variation in the ZmPIMT1 promoter enhances seed aging tolerance by regulating PABP2 repair in maize.The Plant cell · 2025Article
- Identification of the ZmATG18 subfamily genes in maize and the role of ZmATG18a in drought stress.BMC genomics · 2025Article
- Examination of gene expression inmicroPublication biology · 2025Article
- The Dynamic Effects of Transposon Insertions onmicroPublication biology · 2025Article
- Brassinosteroid biosynthesis and signaling: Conserved and diversified functions of core genes across multiple plant species.Plant communications · 2024Review
- COI1 F-box proteins regulate DELLA protein levels, growth, and photosynthetic efficiency in maize.The Plant cell · 2024Article
- ZmPILS6 is an auxin efflux carrier required for maize root morphogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- The maizePlant direct · 2024Article
- Independent evolution of transposase and TIRs facilitated by recombination betweenProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Maize PPR-E proteins mediate RNA C-to-U editing in mitochondria by recruiting the trans deaminase PCW1.The Plant cell · 2023Article
- Root angle in maize influences nitrogen capture and is regulated by calcineurin B-like protein (CBL)-interacting serine/threonine-protein kinase 15 (ZmCIPK15).Plant, cell & environment · 2022Article
- Retrotransposons: How the continuous evolutionary front shapes plant genomes for response to heat stress.Frontiers in plant science · 2022Review
- Evaluation of genome and base editing tools in maize protoplasts.Frontiers in plant science · 2022Article
- A mixed-linkage (1,3;1,4)-β-D-glucan specific hydrolase mediates dark-triggered degradation of this plant cell wall polysaccharide.Plant physiology · 2021Article
4 more citing papers are in PubMed but not listed here.
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Authors and funding
21 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundGene knockouts are a critical resource for functional genomics. In Arabidopsis, comprehensive knockout collections were generated by amplifying and sequencing genomic DNA flanking insertion mutants. These Flanking Sequence Tags (FSTs) map each mutant to a specific locus within the genome. In maize, FSTs have been generated using DNA transposons. Transposable elements can generate unstable insertions that are difficult to analyze for simple knockout phenotypes. Transposons can also generate somatic insertions that fail to segregate in subsequent generations.
resultsTransposon insertion sites from 106 UniformMu FSTs were tested for inheritance by locus-specific PCR. We confirmed 89% of the FSTs to be germinal transposon insertions. We found no evidence for somatic insertions within the 11% of insertion sites that were not confirmed. Instead, this subset of insertion sites had errors in locus-specific primer design due to incomplete or low-quality genomic sequences. The locus-specific PCR assays identified a knockout of a 6-phosphogluconate dehydrogenase gene that co-segregates with a seed mutant phenotype. The mutant phenotype linked to this knockout generates novel hypotheses about the role for the plastid-localized oxidative pentose phosphate pathway during grain-fill.
conclusionWe show that FSTs from the UniformMu population identify stable, germinal insertion sites in maize. Moreover, we show that these sequence-indexed mutations can be readily used for reverse genetic analysis. We conclude from these data that the current collection of 1,882 non-redundant insertion sites from UniformMu provide a genome-wide resource for reverse genetics.
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