ArticleTraffic (Copenhagen, Denmark)2007
A GAPDH mutant defective in Src-dependent tyrosine phosphorylation impedes Rab2-mediated events.
Article in Traffic (Copenhagen, Denmark), 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 57 citations in OpenAlex.
- The role of the Src family kinase Lyn in the immunomodulatory activities of cathelicidin peptide LL-37 on monocytic cells.Journal of leukocyte biology · 2012Trial
- Glyceraldehyde-3-phosphate dehydrogenase 7 (FtGAPDH7) confers heat stress tolerance in buckwheat [Fagopyrum tataricum (L.) Gaertn].Plant cell reports · 2025Article
- Src: coordinating metabolism in cancer.Oncogene · 2022Review
- Rab proteins as major determinants of the Golgi complex structure.Small GTPases · 2018Review
- Proteome-Wide Characterization of Phosphorylation-Induced Conformational Changes in Breast Cancer.Journal of proteome research · 2018Article
- MAP1203 PromotesFrontiers in cellular and infection microbiology · 2018Article
- Membrane skeletal association and post-translational allosteric regulation of Toxoplasma gondii GAPDH1.Molecular microbiology · 2017Article
- GAPDH binds Akt to facilitate cargo transport in the early secretory pathway.Experimental cell research · 2016Article
- Critical protein GAPDH and its regulatory mechanisms in cancer cells.Cancer biology & medicine · 2015Review
- Bypassing hazard of housekeeping genes: their evaluation in rat granule neurons treated with cerebrospinal fluid of multiple sclerosis subjects.Frontiers in cellular neuroscience · 2015Article
- Rab2A is a pivotal switch protein that promotes either secretion or ER-associated degradation of (pro)insulin in insulin-secreting cells.Scientific reports · 2014Article
- Identification of Mycobacterium avium genes expressed during in vivo infection and the role of the oligopeptide transporter OppA in virulence.Microbial pathogenesis · 2014Article
- Overexpression of atypical protein kinase C in HeLa cells facilitates macropinocytosis via Src activation.Cellular signalling · 2014Article
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- Glyceraldehyde 3-phosphate dehydrogenase is required for band 3 (anion exchanger 1) membrane residency in the mammalian kidney.American journal of physiology. Renal physiology · 2011Article
- GAPDH regulates cellular heme insertion into inducible nitric oxide synthase.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
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- The glyceraldehyde-3-phosphate dehydrogenase and the small GTPase Rab 2 are crucial for Brucella replication.PLoS pathogens · 2009Article
- Proteomic analysis of DNA-protein cross-linking by antitumor nitrogen mustards.Chemical research in toxicology · 2009Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) has multiple intracellular activities in addition to its role in gluconeogenesis. Indeed, we have reported that GAPDH is required for Rab2-mediated retrograde transport from vesicular tubular clusters (VTCs). These diverse GAPDH activities are the result of posttranslational modifications that confer a new function to the enzyme. In that regard, GAPDH is tyrosine phosphorylated by Src. To establish the functional significance of this modification for GAPDH activity in Rab2-dependent events, an amino acid substitution was made at tyrosine 41 (GAPDH Y41F). The inability of Src to phosphorylate purified recombinant GAPDH Y41F was confirmed in an in vitro kinase assay. The mutant was then employed in a quantitative membrane-binding assay that measures Rab2 recruitment of soluble components to VTCs. As we observed with GAPDH wild type, Rab2 promoted GAPDH Y41F binding to membranes in a dose-dependent manner, indicating that GAPDH tyrosine phosphorylation is not required for VTC association. However, GAPDH was tyrosine phosphorylated on VTCs. Importantly, GAPDH Y41F blocked vesicular stomatitis virus-G transport in an assay that reconstitutes endoplasmic reticulum to Golgi trafficking, indicating that phosphorylation of tyrosine 41 is essential for GAPDH activity in the early secretory pathway. The block in transport is because of the decreased binding of atypical protein kinase C iota/lambda to GAPDH Y41F, which reduces beta-coat protein association with the VTC and subsequent formation of Rab2-mediated retrograde vesicles. Our results suggest that Src plays a pivotal role in regulating the interaction of Rab2 effectors on the VTC.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.